1887

Abstract

SUMMARY: Syntrophism was demonstrated between strains of and in a chemically defined medium which lacked phenylalanine and pteroylglutamic acid (PGA); however, the presence of -aminobenzoic acid (PABA) was essential. Enumeration of the two organisms in syntrophic growth was found to be possible by the use of Trypticase soy agar + 0·25% (w/v) glucose at pH 9·0 as a selective medium for . It was found that predominated in the early stages of the syntrophic growth, but that eventually predominated. Characterization by a bioautographic technique of the phenylalanine-active factor synthesized by and required by indicated that the factor was not identical with phenylalanine or shikimic acid. Micro-biological assays of the culture fluid from cultures of indicated that three types of folic acid compounds were synthesized: (1) oxygen-stable compounds which supported the growth of ; (2) oxygen-labile compounds which supported the growth of ; (3) oxygen-stable compounds which supported the growth of but not of .

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/content/journal/micro/10.1099/00221287-25-1-77
1961-05-01
2022-01-21
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