Electrophoretic, Sedimentation and Diffusion Behaviour of Crystalline Neuraminidase from Vibrio cholerae

J. Pye; C. C. Curtain

doi:10.1099/00221287-24-3-423

Electrophoretic, Sedimentation and Diffusion Behaviour of Crystalline Neuraminidase from Vibrio cholerae

J. Pye¹ and C. C. Curtain²
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¹ The Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital Post Office, Victoria, Australia ² Baker Medical Research Institute, Alfred Hospital, Melbourne, Victoria, Australia
Published: 01 March 1961 https://doi.org/10.1099/00221287-24-3-423

Abstract

Summary: Crystalline neuraminidase prepared from Vibrio cholerae culture fluids by Ada, French & Lind (1961) was examined by moving boundary electrophoresis, analytical ultracentrifugation and diffusion. The various corresponding physical constants were obtained. The results indicated that, within the limits of the methods used, the crystalline protein was enzyme and was homogeneous.

Received: 21/10/1960
Published Online: 01/03/1961

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References

Ada G. L., French E. L., Lind P. E. 1961; Purification and properties of neuraminidase from Vibrio cholerae . J. gen. Microbiol 24:409
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French E. L., Ada G. L. 1959; Stimulation of the production of neuraminidase in Vibrio cholerae cultures by N-acetylneuraminic acid and sialyl lactose. J. gen. Microbiol 21:550
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Moore D. H., Opperman K. 1956; Adaptation of combined interferometric and phase-plate gradient optics to electrophoresis. Biochim. biophys. Acta 22:136
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Schramm G., Mohr E. 1959; Purification of neuraminidase from Vibrio cholerae . Nature; Lond: 1831677
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Electrophoretic, Sedimentation and Diffusion Behaviour of Crystalline Neuraminidase from Vibrio cholerae

Microbiology 24, 423 (1961); https://doi.org/10.1099/00221287-24-3-423

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Microbiology

Volume 24, Issue 3

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Electrophoretic, Sedimentation and Diffusion Behaviour of Crystalline Neuraminidase from Vibrio cholerae

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