1887

Abstract

Summary: The formation and role of enzyme, virolysin, in K infected with phages P and P are described. Virolysin is a by-product of the metabolism of the cell which is actively producing phage, not of the normal cell. Virolysin is first detected within 10-15 min. in a 40–50 min. latent period and increases linearly until lysis. Normal cell autolysin remains constant during infection. Observations on lysis and phage release show that (1) certain inhibitors which prevent lysis of the cocci by external virolysin also prevent lysis and phage release when added at the end of the latent period; (2) the rate of premature lysis of, and phage release from, cocci chilled during the latent period depends upon their virolysin content. Both observations suggest that virolysin functions in phage release.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-24-3-313
1961-03-01
2022-01-17
Loading full text...

Full text loading...

/deliver/fulltext/micro/24/3/mic-24-3-313.html?itemId=/content/journal/micro/10.1099/00221287-24-3-313&mimeType=html&fmt=ahah

References

  1. Adams M. H., Park B. H. 1956; An enzyme produced by a phage-host cell system. II. The properties of the polysaccharide depolymerase. Virology 2:719
    [Google Scholar]
  2. Bronfenbrenner J., Muckenfuss R. 1927; Studies on the bacteriophage d’Herelle. VIII. The mechanism of lysis of dead bacteria in the presence of bacteriophage. J. exp. Med 45:887
    [Google Scholar]
  3. Flaks J. G., Cohen S. S. 1959; Virus-induced acquisition of metabolic function. I. Enzymatic formation of 5-hydroxymethyldeoxycytidylate. J. biol. Chem 234:1501
    [Google Scholar]
  4. Flaks J. G., Lichtenstein J., Cohen S. S. 1959; Virus-induced acquisition of metabolic function. II. Studies on the origin of the deoxycytidylatehydroxymethylase of bacteriophage-infected E. coli . J. biol. Chem 234:1507
    [Google Scholar]
  5. Fong J., Krueger A. P. 1950; The lytic action of tyrothricin and its derivatives on Staphylococcus aureus . J. gen. Physiol 33:311
    [Google Scholar]
  6. Gottschalk A. 1952; Carbohydrate residue of a urine mucoprotein inhibiting influenza virus haemagglutination. Nature; Lond: 170662
    [Google Scholar]
  7. Gottschalk A. 1958; The prosthetic group of some mucoproteins and its relationship to influenza virus. In Chemistry and Biology of Mucopolysaccharides p. 287 Wolstenholme G. E. W., O’Connor M. Ed Boston: Little, Brown and Co;
    [Google Scholar]
  8. Hancock R., Park J. T. 1958; Cell-wall synthesis by Staphylococcus aureus in the presence of chloramphenicol. Nature; Lond: 1811050
    [Google Scholar]
  9. Humphries J. C. 1948; Enzymic activity of bacteriophage-culture lysates. I. A capsule lysin active against Klebsiella pneumoniae type A. J. Bact 56:683
    [Google Scholar]
  10. Jones D., Krueger A. P. 1951; A rapid slide plaque technic for bacteriophage assay. J. gen. Physiol 34:347
    [Google Scholar]
  11. Koch G., Dreyer W. J. 1958; Characterization of an enzyme of phage T2 as a lysozyme. Virology 6:291
    [Google Scholar]
  12. Kornberg A., Zimmerman S. B., Kornberg S. R., Josse J. 1959; Enzymatic synthesis of DNA. II. Influence of phage T2 on the synthetic pathway in host cells. Proc. nat. Acad. Sci., Wash 45:772
    [Google Scholar]
  13. Kosloff L. M. 1953; Origin and fate of bacteriophage material. Cold Spr. Harb. Symp. quant. Biol 18:209
    [Google Scholar]
  14. Kunkee R. E., Pardee A. B. 1956; Studies on the role of desoxyribonuclease in T2 bacteriophage development. Biochim. biophys. Acta 19:236
    [Google Scholar]
  15. Lieberman M. 1956; Aspects of lysis in normal and phage-infected staphylococci. Ph.D. Thesis University of California, Berkeley:
    [Google Scholar]
  16. Maxted W. R. 1957; The active agent in nascent phage lysis of streptococci. J. gen. Microbiol 16:584
    [Google Scholar]
  17. Mommaerts E. B., Eckert E. A., Beard D., Sharp D. G., Beard J. W. 1952; Dephosphorylation of adenosine triphosphate by concentrates of the virus of avian erythromyeloblastic leucosis. Proc. Soc. exp. Biol., N.Y 79:450
    [Google Scholar]
  18. Murphy J. S. 1957; A phage-associated enzyme of Bacillus megaterium which destroys the bacterial cell wall. Virology 4:563
    [Google Scholar]
  19. Murphy J. S. 1960; An agent derived from B. megaterium phage G which dissolves the bacterial cell wall. Virology 11:510
    [Google Scholar]
  20. Panijel J., Huppert J. 1954; Mise en évidence et titration d’une substance lytique non reproductible en série (lysine) accompagnant la production d’un bactériophage. C.R. Acad. Sci., Paris 238:745
    [Google Scholar]
  21. Pardee A. B., Jacob F., Monod J. 1958; Sur l’expression et le rôle des allèles ‘inductible’ et ‘constitutive’dans la synthèse de la β-galactosidase des zygotes d’Escherichia coli . C.R. Acad. Sci., Paris 246:3125
    [Google Scholar]
  22. Ralston D. J., Baer B. 1960; Improved method of phage assay by a plaque count method on microscope slides in trays. J. Bact 79:758
    [Google Scholar]
  23. Ralston D. J., Krueger A. P. 1952; Phage multiplication on two hosts.Isolation and activity of variants of staphylococcus phage P1 . Proc. Soc. exp. Biol., N.Y 80:217
    [Google Scholar]
  24. Ralston D. J., Krueger A. P. 1954; The isolation of a staphylococcal phage variant susceptible to an unusual host control. J. gen. Physiol 37:685
    [Google Scholar]
  25. Ralston D. J., Baer B., Krueger A. P. 1955a; Lytic factor found in staphylococcal bacteriophage lysates. Fed. Proc 14:475
    [Google Scholar]
  26. Ralston D. J., Baer B., Lieberman M., Krueger A. P. 1955b; Virolysin: a virus-induced lysin from staphylococcal phage lysates. Proc. Soc. exp. Biol., N.Y 89:502
    [Google Scholar]
  27. Ralston D. J., Baer B., Lieberman M., Krueger A. P. 1957a; Lysis from without of S. aureus K1 by the combined action of phage and virolysin. J. gen. Physiol 41:343
    [Google Scholar]
  28. Ralston D. J., Lieberman M., Baer B., Krueger A. P. 1957b; Staphylococcal virolysin, a phage-induced lysin. Its differentiation from the autolysin of normal cells. J. gen. Physiol 40:791
    [Google Scholar]
  29. Sertic V. 1929; Untersuchungen über einen Lysinzonen bildenden Bakteriophagen. I. Mitteilung: Der Aufbau der Bakteriophagenkolonien. Zbl. Bakt Abt. 1 110:125
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-24-3-313
Loading
/content/journal/micro/10.1099/00221287-24-3-313
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error