1887

Abstract

SUMMARY: When organisms were suspended in distilled water and freeze-dried the maximum loss of viability did not occur during the drying process proper, but during the time of contact of the dried organisms with air between the primary and the secondary drying periods. By substituting other gases for air at this stage, it was proven that oxygen was the active agent involved. The dried organisms which were exposed to different pressures of air and oxygen at different temperatures proved to be extremely sensitive to traces of oxygen, even at very low temperatures. The implications of this oxygen effect in connexion with existing freeze-drying procedures, as well as some preliminary kinetic experiments concerning the shape of the survival curve, are discussed.

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/content/journal/micro/10.1099/00221287-24-2-191
1961-02-01
2024-04-25
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References

  1. Collier L. H. 1954 In A Discussion on the Maintenance of Cultures by Freeze-Drying p. 41 London: H.M. Stationery Office;
    [Google Scholar]
  2. Fieser L. F. 1924; A new absorbant for oxygen in gas analysis. J. Amer. chem. Soc 46:2639
    [Google Scholar]
  3. Fry R. M. 1954; The preservation of bacteria. In Biological Applications of Freezing and Drying p. 215 Harris R. J. C. Ed New York: Academic Press Inc;
    [Google Scholar]
  4. Fry R. M., Greaves R. I. N. 1951; The survival of bacteria during and after drying. J. Hyg., Camb 49:220
    [Google Scholar]
  5. Gard S. 1957; Chemical inactivation of viruses. In The Nature of Viruses; Ciba Symposium p. 123
    [Google Scholar]
  6. Harris R. J. C. 1954 Biological Applications of Freezing and Drying New York: Academic Press Inc;
    [Google Scholar]
  7. Mazur P. 1959; Physical factors implicated in the death of microorganisms at subzero temperatures. Ann. N.Y. Acad. Sci 85:610
    [Google Scholar]
  8. Meryman H. T. 1959; Sublimation freeze-drying without vacuum. Science 130:628
    [Google Scholar]
  9. Morton M. E., Pulaski E. J. 1938; The preservation of bacterial cultures. J. Bact 35:163
    [Google Scholar]
  10. Naylor H. B., Smith P. A. 1946; Factors affecting the viability of Serratia marcescens during dehydration and storage. J. Bact 52:565
    [Google Scholar]
  11. Rogers L. A. 1914; The preparation of dried cultures. J. infect. Dis 14:100
    [Google Scholar]
  12. Stillman F. G. 1941; The preservation of Pneumococcus by freezing and drying. J. Bact 42:689
    [Google Scholar]
  13. Swift H. F. 1937; A simple method for preserving bacterial cultures by freezing and drying. J. Bact 33:411
    [Google Scholar]
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