1887

Abstract

SUMMARY

A procedure is described for measuring the viabilities (% viable/total organisms) of populations of by short-term incubation on agar films followed by differential counting under the microscope. The only special apparatus needed is a supply of metal annuli. The procedure estimates viability with 95 % fiducial limits of ± 9 %; it appears applicable to other aerobic bacteria, to yeasts and to spore suspensions.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-24-1-15
1961-01-01
2022-01-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/24/1/mic-24-1-15.html?itemId=/content/journal/micro/10.1099/00221287-24-1-15&mimeType=html&fmt=ahah

References

  1. Barer R., Ross K. F. A., Tkaczyk S. 1953; Refractometry of living cells. Nature; Lond: 171720
    [Google Scholar]
  2. Firkhman B. A. 1959; The principles of immersion micro-refractometry for a direct determination of live and dead bacterial cells. C.R. Acad. Sci., U.S.S.R 124:1141
    [Google Scholar]
  3. Greenberg L., Eidus L., Diena B. B. 1958; The vital stainjng method for the rapid estimation of the bacterial count of B.C.G vaccine. Amer. Rev. Tuberc 78:785
    [Google Scholar]
  4. Herbert D., Elsworth R., Telling R. C. 1956; The continuous culture of bacteria; a theoretical and experimental study. J. gen. Microbiol 14:601
    [Google Scholar]
  5. Knaysi G. 1935; A microscopic method of distinguishing dead from living bacterial cells. J. Bact 30:193
    [Google Scholar]
  6. Knaysi G., Ford M. 1938; A method of counting viable bacteria in milk by means of the microscope. J. Dairy Sci 21:129
    [Google Scholar]
  7. Knöll H. 1944; Zur Anwendung der Phasenkontrastmikroskopie in der Bakteriologie. Zeiss Nachr 5:38
    [Google Scholar]
  8. Koch A. L. 1959; Death of bacteria in growing culture. J. Bact 77:623
    [Google Scholar]
  9. Mager J., Kuczynski M., Schatzberg G., Avi-Dor Y. 1956; Turbidity changes in bacterial suspensions in relation to osmotic pressure. J. gen. Microbiol 14:69
    [Google Scholar]
  10. Powell E. O. 1956; A rapid method for determining the proportion of viable bacteria in a culture. J. gen. Microbiol 14:153
    [Google Scholar]
  11. Razumovskaya Z. G., Osipova I. V. 1958; The ratio between live and dead bacteria in a multiplying culture of Acetobader melanogenum . Microbiologiya 27:727
    [Google Scholar]
  12. Strugger S. 1948; Fluorescence microscope examination of bacteria in soil. Canad. J. Res 26:188
    [Google Scholar]
  13. Taubeneck U. 1957; Die Herstellung von Formvar-Netzfolien und ihreVerwendung für Mikrokulturen. Z. Bakt 1 168:627
    [Google Scholar]
  14. Taubeneck U. 1959; New grid-replica for precise localization in slide culture. J. Bact 77:506
    [Google Scholar]
  15. Townsend G. F., Lindegren C.C. 1953; Structures in the yeast cell revealed in wet mounts. Cytologia, Tokyo 18:183
    [Google Scholar]
  16. Valentine R. C., Bradfield J. R. G. 1954; The urea method for bacterial viability counts with the electron microscope and its relation to other viability counting methods. J. gen. Microbiol 11:349
    [Google Scholar]
  17. Wade H. E., Morgan D. M. 1954; Differentiation of growing and non-growing bacteria by a staining technique. Nature; Lond: 174920
    [Google Scholar]
  18. White P. B. 1947; A method for combined positive and negative staining of bacteria. J. Path. Bact 59:334
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-24-1-15
Loading
/content/journal/micro/10.1099/00221287-24-1-15
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error