1887

Abstract

SUMMARY: A method is described which is suitable for routine viable counts of rumen bacteria capable of fermenting various carbohydrates. The contribution of different steps in the preparation of the medium towards the final redox potential was determined and the choice of a suitable reducing agent, redox indicator and source of rumen fluid supplement is discussed. In a sheep on a constant practical ration, the variation in numbers of cellulolytic bacteria was greater than the differences between five successive samples withdrawn at one time, and much greater than the variation due to the method.

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/content/journal/micro/10.1099/00221287-23-3-565
1960-12-01
2021-07-30
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References

  1. Briggs C.A.E. 1955; Viable bacteria in the rumen. Dairy Sci. Abstr 17:712
    [Google Scholar]
  2. Bryant M.P. 1959; Bacterial species of the rumen. Bact. Rev 23:125
    [Google Scholar]
  3. Bryant M.P., Burkey L.A. 1953a; Cultural methods and some characteristics of some of the more numerous groups of bacteria in the bovine rumen. J. Dairy Sci 36:205
    [Google Scholar]
  4. Bryant M.P., Burkey L.A. 1953b; Numbers and some predominant groups of bacteria in the rumen of cows fed different rations. J. Dairy Sci 36:218
    [Google Scholar]
  5. Cook A.M., Steel K.J. 1959; A method for the preparation of bacterial dilutions. Lab. Pract 8:359
    [Google Scholar]
  6. Elsden S.R., Phillipson A.T. 1948; Ruminant digestion. Anna. Rev. Biochem 17:705
    [Google Scholar]
  7. Gall L.S., Huhtanen C.N. 1951; Criteria for judging a true rumen organism and a description of five rumen bacteria. J. Dairy Sci 34:353
    [Google Scholar]
  8. Gilchrist F.M.C., Clark R. 1957; Refresher courses in physiology. III. The microbiology of the rumen. J. S. Afr. vet. med. Ass 28:295
    [Google Scholar]
  9. Huhtanen C.N., Gall L.S. 1953; Rumen organisms. I. Curved rods and a related rod type. J. Bact 65:548
    [Google Scholar]
  10. Huhtanen C.N., Rogers M.R., Gall L.S. 1952; Improved techniques for isolating and purifying rumen organisms. J. Bact 64:17
    [Google Scholar]
  11. Hungate R.E. 1950; The anaerobic mesophilic cellulolytic bacteria. Bact. Rev 14:1
    [Google Scholar]
  12. Pearson R.M., Smith J.A.B. 1943; Utilization of urea in bovine rumen. I. Methods of analysis of rumen ingesta and preliminary experiments in vivo . Biochem. J 37:142
    [Google Scholar]
  13. Smith P.H., Hungate R.E. 1958; Isolation and characterization of Methanobaderium ruminantium n.sp. J. Bact 75:713
    [Google Scholar]
  14. Smith P.H., Sweeney H.C., Rooney J.R., King K.W., Moore W.E.C. 1956; Stratification and kinetic changes in the ingesta of the bovine rumen. J. Dairy Sci 39:598
    [Google Scholar]
  15. Wilson M.K., Briggs C.A.E. 1955; The normal flora of the bovine rumen. II. Quantitative bacteriological studies. J. appl. Bact 18:294
    [Google Scholar]
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