1887

Microbiology Society logo

Volume 23, Issue 3

The Formation of Ribulose 1:5-Diphosphate Carboxylase by Growing Cultures of Athiorhodaceae Free

Abstract

Summary: Ribulose l:5-diphosphate carboxylase, which catalyses the conversion of ribulose l:5-diphosphate and CO to 3-phosphoglyceric acid, is a key enzyme in the reductive pentose cycle. It was formed only in traces by growing in the dark under conditions of high or low aeration but organisms grown photosynthetically were rich in the enzyme. The activity of some other enzymes of the cycle, phosphoriboisomerase, phosphoglyceric acid kinase and triosephosphate dehydrogenase (diphosphopyridine nucleotide-linked) did not vary significantly with the growth conditions. The differential rate of synthesis (increase in enzyme/ increase in culture density) of the carboxylase was studied in cultures of growing exponentially under various conditions. It was influenced by the light intensity, being decreased when this was increased. Oxygen completely repressed formation of the enzyme even under continuous illumination; this effect was annulled by restoration of anaerobic conditions. Dark-grown organisms formed the enzyme at a high differential rate immediately on transfer to anaerobic + light conditions, provided that they contained a minimal amount of bacteriochlorophyll. Biotin deficiency did not affect the synthesis of the carboxylase. The conditions which promoted carboxylase formation were in many respects similar to those favourable to synthesis of enzymes required for the early stages of bacteriochlorophyll formation. The connexion of these observations with the regulation of pigment synthesis and with the possible physiological role of the enzyme in the Athiorhodaceae is discussed.

  • Received:
  • Published Online:
© Society for General Microbiology 1960
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-23-3-499
1960-12-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/23/3/mic-23-3-499.html?itemId=/content/journal/micro/10.1099/00221287-23-3-499&mimeType=html&fmt=ahah

References

  1. Arnon D.I., Rosenberg L.L., Whatley F.R. 1954; A new glyceraldehyde phosphate dehydrogenase from photosynthetic tissues. Nature; Lond.: 1731132
     [Google Scholar]
  2. Aubert J.-P., Milhaud G., Millet J. 1957; L’Assimilation de l’anhydride carbonique par les bactéries chimioautotrophes. Ann. Inst.Pasteur 92:515
     [Google Scholar]
  3. Bassham J.A., Calvin M. 1957 The Path of Carbon in Photosynthesis Englewood Cliffs, N.J.: Prentice-Hall, Inc;
     [Google Scholar]
  4. Bergmann F.H., Towne J.C., Burris R.H. 1958; Assimilation of carbon dioxide by hydrogen bacteria. J. biol. Chem 230:13
     [Google Scholar]
  5. Bücher T. 1947; Über ein phosphatübertragendes Gärungsferment. Biochim. biophys.Acta 1:292
     [Google Scholar]
  6. Cohen-Bazire G., Sistrom W.R., Stanier R.Y. 1957; Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J. cell. comp. Physiol 49:25
     [Google Scholar]
  7. Cutinelli C., Ehrensvärd G., Reio L., Saluste E., Stjernholm R. 1951; Acetic acid metabolism in Rhodospirillum rubrum under anaerobic conditions 2. Ark.Kemi 3:315
     [Google Scholar]
  8. Frenkel A.W. 1959; Light-induced reactions of bacterial chromatophores and their relation to photosynthesis. Annu. Rev. Plant Physiol 10:53
     [Google Scholar]
  9. Fuller R.C., Gibbs M. 1959; Intracellular and phylogenetic distribution of ribulose l:5-diphosphate carboxylase and d-glyceraldehyde 3-phosphate dehydrogenases. Plant Physiol 34:324
     [Google Scholar]
  10. Gaffron H. 1933; Über den Stoffwechsel der Schwefelfreien Purpurbakterien. 1. Bio-chem.Z 260:1
     [Google Scholar]
  11. Gaffron H. 1935; Über den Stoffwechsel der Purpurbakterien. 2. Biochem. Z 275:301
     [Google Scholar]
  12. Gibbs M. 1952; Triosephosphate dehydrogenase and glucose-6-phosphate dehydrogenase in the pea plant. Nature; Lond.: 170164
     [Google Scholar]
  13. Glover J., Kamen M.D., Van Genderen H. 1952; Studies on the metabolism of photosynthetic bacteria. XII. Comparative light and dark metabolism of acetate and carbonate by Rhodospirillum rubrum. . Arch. Biochem 35:384
     [Google Scholar]
  14. Horecker B.L., Hurwitz J., Weissbach A. 1958; Ribulose diphosphate. Biochemical Preparations 683 Vestling C.S. Ed. by New York: John Wiley and Sons, Inc;
     [Google Scholar]
  15. Kornberg H.L., Collins J.F., Bigley D. 1960; The influence of growth substrates on metabolic pathways in Micrococcus denitrificans. . Biochim. biophys.Acta 39:9
     [Google Scholar]
  16. Lascelles J. 1956; The synthesis of porphyrins and bacteriochlorophyll by cell suspensions of Rhodopseudomonas spheroides. . Biochem. J 62:78
     [Google Scholar]
  17. Lascelles J. 1959; Adaptation to form bacteriochlorophyll in Rhodopseudomonas spheroides : changes in activity of enzymes concerned in pyrrole synthesis. Biochem. J 72:508
     [Google Scholar]
  18. Lascelles J. 1960; The synthesis of enzymes concerned in bacteriochlorophyll formation in growing cultures of Rhodopseudomonas spheroides. . J. gen. Microbiol 23:487
     [Google Scholar]
  19. Lynen F., Knappe J., Lorch E., Jütting G., Ringelmann E. 1959; Die biochemische Funktion des Biotins. Angew. Chem 71:481
     [Google Scholar]
  20. Monod J., Pappenheimer A.M. Jr. Cohen-Bazire G. 1952; La cinétique de la biosynthèse de la β-galactosidase chez Escherichia coli considérée comme fonction de la croissance. Biochim. biophys.Acta 9:648
     [Google Scholar]
  21. Niel C.B.van. 1941; The bacterial photosynth�ses and their importance for the general problem of photosynthesis. Advanc. Enzymol 1:263
     [Google Scholar]
  22. Niel C.B.van. 1944; The culture, general physiology, morphology and classification of the non-sulfur purple and brown bacteria. Bact. Rev 8:1
     [Google Scholar]
  23. Olson J.M., Duysens L.N.M., Kronenberg G.H.M. 1959; Spectrofluorometry of pyridine nucleotide reactions in Chromatium. . Biochim. biophys.Acta 36:125
     [Google Scholar]
  24. Olson J.M., Amesz J. 1960; Action spectra for fluorescence excitation of pyridine nucleotide in photosynthetic bacteria and algae. Biochim. biophys. Acta 37:14
     [Google Scholar]
  25. Ormerod J.G. 1956; The use of radioactive carbon dioxide in the measurement of carbon dioxide fixation in Rhodospirillum rubrum. . Biochem. J 64:373
     [Google Scholar]
  26. Packer L. 1958; Respiratory carriers involved in the oxidation of hydrogen and lactate in a facultative autotroph. Arch. Biochem. Biophys 78:54
     [Google Scholar]
  27. Quayle J.R., Fuller R.C., Benson A.A., Calvin M. 1954; Enzymatic carboxylation of ribulose diphosphate. J. Amer. chem. Soc 76:3610
     [Google Scholar]
  28. Quayle J.R., Keech D.B. 1959a; Carbon assimilation by Pseudomonas oxalaticus (OX1). 1. Formate and carbon dioxide utilization during growth on formate. Biochem. J 72:623
     [Google Scholar]
  29. Quayle J.R., Keech D.B. 1959b; Carbon assimilation by Pseudomonas oxalaticus (OX1). 2. Formate and carbon dioxide utilization by cell-free extracts of the organism grown on formate. Biochem. J 72:631
     [Google Scholar]
  30. Quayle J.R., Keech D.B. 1960; Carbon assimilation by Pseudomonas oxalaticus (OX1). 3. Oxalate utilization during growth on oxalate. Biochem. J 75:515
     [Google Scholar]
  31. Racker E. 1957; The reductive pentose cycle. 1. Phosphoribulokinase and ribulose diphosphate carboxylase. Arch. Biochem. Biophys 69:300
     [Google Scholar]
  32. Smith L., Ramírez J. 1959; Absorption spectrum changes in photosynthetic bacteria following illumination or oxygenation. Arch. Biochem. Biophys 79:233
     [Google Scholar]
  33. Smith L., Baltscheffsky M., Olson J.M. 1960; Adsorption spectrum changes observed on illumination of aerobic suspensions of photosynthetic bacteria. J. biol. Chem 235:213
     [Google Scholar]
  34. Stanier R.Y., Doudoroff M., Kunisawa R., Contopoulou R. 1959; The role of organic substrates in bacterial photosynthesis. Proc. nat. Acad. Sci., Wash 45:1246
     [Google Scholar]
  35. Stoppani A.O.M., Fuller R.C., Calvin M. 1955; Carbon dioxide fixation by Rhodopseudomonas capsulatus. . J. Bact 69:491
     [Google Scholar]
  36. Trudinger P.A. 1956; Fixation of carbon dioxide by extracts of the strict autotroph Thiobadllus denitrificans. . Biochem. J 64:274
     [Google Scholar]
  37. Vishniac W., Santer M. 1957; The Thiobacilli. Bact. Rev 21:195
     [Google Scholar]
  38. Weissbach A., Smyrniotis P.Z., Horecker B.L. 1954; Pentose phosphate and C02 fixation in spinach extracts. J. Amer. chem. Soc 76:3611
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-23-3-499
Loading
The Formation of Ribulose 1:5-Diphosphate Carboxylase by Growing Cultures of Athiorhodaceae
Microbiology 23, 499 (1960); https://doi.org/10.1099/00221287-23-3-499
/content/journal/micro/10.1099/00221287-23-3-499
/content/journal/micro/10.1099/00221287-23-3-499
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error