1887

Abstract

Summary: The enzymes -aminolaevulic acid synthetase and <5-aminolaevulic acid dehydrase are concerned in the early stages of tetrapyrrole formation; factors controlling their synthesis have been studied in cultures of growing exponentially. In organisms growing in the dark under high degrees of aeration the differential rate of synthesis (increase in enzyme/increase in culture density) of both enzymes is about one-third of that in cultures growing anaerobically in the light. Organisms growing in the dark under low degrees of aeration form the enzymes at rates comparable to those in photosynthetic cultures. Under anaerobic conditions the differential rate of synthesis of both enzymes is decreased by increasing the light intensity. Enzyme synthesis in the light is repressed by oxygen, the effect being overcome on restoration of anaerobic conditions. Formation of bacterio- chlorophyll under these various conditions is affected in the same way as enzyme synthesis. Addition of -aminolaevulic acid or haemin to growing cultures stopped the synthesis of the synthetase and the dehydrase; magnesium protoporphyrin had no such effect. The activity of the synthetase, but not of the dehydrase, was decreased in organisms growing with suboptimal concentrations of biotin. A component of the electron transport chain as well as the intracellular concentration of biosynthetic intermediates may contribute, by mechanisms as yet unknown, to the regulation of synthesis of enzymes involved in bacteriochlorophyll formation.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-23-3-487
1960-12-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/23/3/mic-23-3-487.html?itemId=/content/journal/micro/10.1099/00221287-23-3-487&mimeType=html&fmt=ahah

References

  1. Ames B.N., Garry B. 1959; Coordinate repression of the synthesis of four histidine biosynthetic enzymes by histidine. Proc. nat. Acad. Sci., Wash 45:1453
    [Google Scholar]
  2. Cohen-Bazire G., Sistrom W.R., Stanier R.Y. 1957; Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J. cell. comp. Physiol 49:25
    [Google Scholar]
  3. Cooper R. 1956; The production of coproporphyrin precursors by a Rhodopseudomonas sp. . Biochem. J 63:25P
    [Google Scholar]
  4. Falk J.E., Porra R.J., Brown A., Moss F., Larminie H.E. 1959; Effect of oxygen tension on haem and porphyrin biosynthesis. Nature; Lond.: 1841217
    [Google Scholar]
  5. Gibson K.D. 1958; Biosynthesis of δ-aminolaevulic acid by extracts of Rhodo-pseudomonas spheroides. . Biochim. biophys. Acta 28:451
    [Google Scholar]
  6. Granick S. 1948; Magnesium protoporphyrin as a precursor of chlorophyll in Chlorella. J. biol. Chem 175:333
    [Google Scholar]
  7. Heath H., Hoare D.S. 1959; The biosynthesis of porphyrins from porphobilinogen by Rhodopseudomonas spheroides. . Biochem. J 72:14
    [Google Scholar]
  8. Heyningen W.E.Van, Gladstone G.P. 1953; The neurotoxin of Shigella shigae. 3. The effect of iron on production of the toxin. Brit. J. exp. Path 34:221
    [Google Scholar]
  9. Hoare D.S., Heath H. 1959; The biosynthesis of porphyrins from porphobilinogen by Rhodopseudomonas spheroides. 2. The partial purification and some properties of porphobilinogen deaminase and uro-porphyrinogen decarboxylase. Biochem. J 73:679
    [Google Scholar]
  10. Hutner S.H. 1950; Anaerobic and aerobic growth of purple bacteria (Athiorhodaceae) in chemically defined media. J. gen. Microbiol 4:286
    [Google Scholar]
  11. Kikuchi G., Kumar A., Shemin D. 1959; Mechanism of enzymatic synthesis of δ-aminolevulinic acid. Fed. Proc 18:259
    [Google Scholar]
  12. Kikuchi G., Kumar A., Talmage P., Shemin D. 1958; The enzymatic synthesis of δ-aminolevulinic acid. J. biol. Chem 233:1214
    [Google Scholar]
  13. LascellES J. 1956a; The synthesis of porphyrins and bacteriochlorophyll by cell suspensions of Rhodopseudomonas spheroides. . Biochem. J 62:78
    [Google Scholar]
  14. Lascelles J. 1956b; An assay of iron protoporphyrin based on the reduction of nitrate by a variant strain of Staphylococcus aureus: synthesis of iron protoporphyrin by suspensions of Rhodopseudomonas spheroides. . J. gen. Microbiol 15:404
    [Google Scholar]
  15. Lascelles J. 1959; Adaptation to form bacteriochlorophyll in Rhodopseudomonas spheroides: changes in activity of enzymes concerned in pyrrole synthesis. Biochem. J 72:508
    [Google Scholar]
  16. Lynen F., Knappe J., Lorch E., Jütting G., Ringelmann E. 1959; Die bio-chemische Funktion des Biotins. Angew. Chem 71:481
    [Google Scholar]
  17. Mauzerall D., Granick S. 1956; The occurrence and determination of δ-amino- laevulic acid and porphobilinogen in urine. J. biol. Chem 219:435
    [Google Scholar]
  18. Monod J., Cohen-Bazire G., Cohn M. 1951; Sur la biosynthèse de la β-galactosidase (lactase) chez Escherichia coli. La specificité de l’induction. Biochim. biophys. Acta 7:585
    [Google Scholar]
  19. Monod J., Pappenheimer A.M. Jr. Cohen-Bazire G. 1952; La cinétique de la biosynthèse de la β-galactosidase chez Escherichia coli considérée comme fonction de la croissance. Biochim. biophys. Acta 9:648
    [Google Scholar]
  20. Niel C.B.VAN. 1944; The culture, general physiology, morphology and classification of the non-sulfur purple and brown bacteria. Bact. Rev 8:1
    [Google Scholar]
  21. Stickland L.H. 1951; The determination of small quantities of bacteria by means of the Biuret reaction. J. gen. Microbiol 5:698
    [Google Scholar]
  22. Vogel H.J. 1957; Repression and induction as control mechanism of enzyme biogenesis. The adaptive formation of acetylornithinase. In The Chemical Basis of Heredity McElroy W.D., Glass B. Ed. by p. 276 Baltimore: Johns Hopkins Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-23-3-487
Loading
/content/journal/micro/10.1099/00221287-23-3-487
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error