1887

Abstract

SUMMARY: Five of eleven different temperate Proteus phages are capable of intrastrain transduction of a streptomycin resistance marker. Interstrain transduction of this marker was achieved with three of the five phages. Nuclear segregation and phenomic lag may both be involved in the delay in phenotypic expression of streptomycin resistance. The one transducing phage investigated appears to possess a normal phage genome in that transductant colonies are lysogenic and show lysogenic conversion, at multiplicities of phage input of less than 0·02, and under conditions where secondary infection on the plates can be excluded. However, under these conditions a few transductant clones were encountered which were not lysogenic. This indicates that the transducing and lysogenizing functions of this phage may be divorced from each other. Ultraviolet irradiation has a differential effect on its transducing and plaque-forming abilities.

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/content/journal/micro/10.1099/00221287-23-3-445
1960-12-01
2021-10-16
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References

  1. Adams M.H. 1956; Methods of study of bacterial viruses. Meth. Med. Res 2:1
    [Google Scholar]
  2. Adams J.N., Luria S.E. 1958; Transduction by bacteriophage P1: abnormal phage function of the transducing particles. Proc. not. Acad. Set., Wash 44:590
    [Google Scholar]
  3. Alexander H.E., Leidy G. 1953; Induction of streptomycin resistance in sensitive Haemophilus influenzae by extracts containing deoxyribonucleic acid from resistant Haemophilus influenzae . J. exp. Med 97:17
    [Google Scholar]
  4. Belyavin G. 1951; Cultural and serological phases of Proteus vulgaris . J. gen. Microbiol 5:197
    [Google Scholar]
  5. Coetzee J.N. 1958; The characterization of a series of Proteus bacteriophages. S. Afr. J. Lab. clin. Med 4:147
    [Google Scholar]
  6. Coetzee J.N. 1959; Some properties of a morphological variant of a strain of Proteus vulgaris . S. Afr. J. lab. clin. Med 5:16
    [Google Scholar]
  7. Coetzee J.N. 1960; Lysogenic conversion in the genus Proteus. . Nature; Lond.: in the Press
    [Google Scholar]
  8. Coetzee J.N., Sacks T.G. 1959; Lysogeny in the genus Proteus . Nature; Lond.: 1841340
    [Google Scholar]
  9. Coetzee J.N., Sacks T.G. 1960a; Intrastrain transduction in Proteus mirabilis . Nature; Lond.: 185869
    [Google Scholar]
  10. Coetzee J.N., Sacks T.G. 1960b; Morphological variants of Proteus hauseri . J. gen. Microbiol 23:209
    [Google Scholar]
  11. Coetzee J.N., Sacks T.G. 1960c; The late sucrose fermenting property of Proteus mirabilis . S. Afr. J. lab. clin. Med 6:49
    [Google Scholar]
  12. Dienes L. 1947; Further observations on the reproduction of bacilli from large bodies in Proteus cultures. Proc. Soc. exp. Biol., N.Y 66:97
    [Google Scholar]
  13. Edwards P.R., Davis B.R., Cherry W.B. 1955; Transfer of antigens by phage lysates with particular reference to the l, w antigens of Salmonella. J. Bact 70:279
    [Google Scholar]
  14. Garen A., Zinder N.D. 1955; Radiological evidence for partial genetic homology between bacteriophage and host bacteria. Virology 1:347
    [Google Scholar]
  15. Hartman P.E. 1957; Transduction: a comparative review. Symposium—The Chemical Basis of Heredity, 1st ed.. p. 408 McElroy W.D., Glass B. Ed. Baltimore: The Johns Hopkins Press;
    [Google Scholar]
  16. Hershey A.D., Kalmanson G., Bronfenbrenner J. 1943; Quantitative methods in the study of the phage-antiphage reaction. J. Immunol 46:267
    [Google Scholar]
  17. Holloway B.W., Monk M. 1959; Transduction in Pseudomonas aeruginosa . Nature; Lond.: 1841426
    [Google Scholar]
  18. Jacob F. 1955; Transduction of lysogeny in Escherichia coli . Virology 1:207
    [Google Scholar]
  19. Krikler M.S. 1953; The serology of Proteus vulgaris. Ph.D.Thesis University of London:
    [Google Scholar]
  20. Lamanna C., Mallette M.F. 1953 Basic Bacteriology, 1st ed.. p. 404 Baltimore: The Williams and Wilkins Company;
    [Google Scholar]
  21. Lederberg J. 1951; Streptomycin resistance: A genetically recessive mutation. J. Bact 61:549
    [Google Scholar]
  22. Lennox E.S. 1955; Transduction of linked genetic characters of the host by bacteriophage Pi. Virology 1:190
    [Google Scholar]
  23. Loutit J.S. 1959; Effect of ultraviolet irradiation on transduction in Pseudomonas aeruginosa . Nature; Lond.: 1841960
    [Google Scholar]
  24. Morse M.L., Lederberg E.M., Lederberg J. 1956a; Transduction in Escherichia coli K-12. Genetics 41:142
    [Google Scholar]
  25. Morse M.L., Lederberg E.M., Lederberg J. 1956b; Transductional heterogenates in Escherichia coli . Genetics 41:758
    [Google Scholar]
  26. Newcombe H.B., Hawirko R. 1949; Spontaneous mutation to streptomycin resistance and dependence in Escherichia coli . J. Bact 57:565
    [Google Scholar]
  27. Starlinger P. 1958; Øber einen Defekt des transduzierenden Salmonella-Phagen P22. Z. Naturforsch 13B:489
    [Google Scholar]
  28. Stocker B.A.D. 1956; Abortive transduction of motility in Salmonella; a nonreplicated gene transmitted through many generations to a single descendant. J. gen. Microbiol 15:575
    [Google Scholar]
  29. Stocker B.A.D. 1959; Phage-mediated transduction. In Recent Progress in Microbiology, 1st. ed.. p. 31 Tunevall G. Ed. Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  30. Watanabe T., Watanabe M. 1959; Transduction of Streptomycin resistance in Salmonellla typhimurium . J. gen. Microbiol 21:16
    [Google Scholar]
  31. Zinder N.D., Lederberg J. 1952; Genetic exchange in Salmonella. J. Bact 64:679
    [Google Scholar]
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