The Keto Acid Metabolism of growing in Submerged Culture Free

Abstract

SUMMARY: The keto acid production of (strain CN2000) growing in submerged culture in complex medium was investigated. Pyruvic acid production followed a pattern in which there was a very rapid production of acid which reached a peak about 12 hr. after inoculation. The acid concentration decreased rapidly after 12 hr. and the final pyruvic acid concentration appeared to be very variable. The source of the pyruvic acid is believed to be lactic acid, the concentration of which decreased rapidly as pyruvic acid increased. The presence in the medium of sufficient iron to abolish toxin production completely did not affect the pattern of pyruvic acid production. α-Ketoglutaric acid production in the cultures appeared to vary in a random manner. strain G12/6 produced a maximum yield of pyruvic acid after only 6 hr. growth but was otherwise similar to strain CN2000. strain SM1 gave a maximum production of pyruvic acid after 24 hr. but in the presence of 3 g. Fe/ml. production was more rapid and was like that found for strain CN2000. No correlation was found between toxin production and the production of pyruvic acid or α-keto glutaric acid.

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/content/journal/micro/10.1099/00221287-23-2-301
1960-10-01
2024-03-28
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References

  1. Barker S. B., Summerson W. H. 1941; The colorimetric determination of lactic acid in biological material. J. biol. Chem 138:535
    [Google Scholar]
  2. Cavallini D., Frontali M. 1954; Determination of keto-acids by paper partition chromatography. Biochim. biophys. Acta 13:439
    [Google Scholar]
  3. Cavallini D., Mandovi B. 1957; The use of formaldehyde to avoid artefacts in the chromatographic determination of keto acids. Clin. Chim.Acta 2:312
    [Google Scholar]
  4. Edwards D. C. 1960; The growth and toxin production of Corynebacterium diphtheriae in submerged culture. J. gen. Microbiol 22:698
    [Google Scholar]
  5. Edwards D. C. P., Seamer P. A. 1960; The uptake of iron by Corynebacterium, diphtheriae growing in submerged culture. J. gen. Microbiol 22:705
    [Google Scholar]
  6. El Hawary M. F. S., Thomson R. H. S. 1953; Separation and estimation of blood keto acids by paper chromatography. Biochem. J 53:340
    [Google Scholar]
  7. Glenny A. T., Okell C. C. 1924; The titration of diphtheria toxin and antitoxin BY flocculation methods. J. Path. Bact 27:187
    [Google Scholar]
  8. Jännes L. 1954; Studies on the terminal oxidative reaction patterns of the carbohydrate metabolism of Corynebacterium diphtheriae . Ann. Acad. Sci.fenn Ser. A, sec.2) 61:1
    [Google Scholar]
  9. Linggood F. V., Matthews A. C., Pinfield S., Pope C. G., Sharland T. R. 1955; Production of diphtheria toxin in submerged culture. Nature; Lond.: 176:1128
    [Google Scholar]
  10. Pope C. G., Smith M. L. 1932; The routine preparation of diphtheria toxin of high value. J. Path. Bact 35:573
    [Google Scholar]
  11. Ramon G. 1922; Floculation dans un melange neutre de toxin-antitoxine diphteriques. C.R. Soc. Biol., Paris 86:661
    [Google Scholar]
  12. Ramon G., Pochon J., Amoureux G. 1941; La production à l’aide d’un nouveau milieu de culture à base de digestion papainique des toxines microbiennes et specialement des toxines diphtériques et staphylococciques destinees à la preparation des anatoxines correspondantes. C.R. Soc. Biol., Paris 135:1502
    [Google Scholar]
  13. Yoneda M., Ishihara H. 1958; Accumulation of a-alanine and of pyruvate in diphtherial culture filtrate and its relation to iron deficiency of medium. Nature; Lond.: 181272
    [Google Scholar]
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