1887

Abstract

SUMMARY: The anaerobic filamentous organism grows as discrete colonies which sediment in static liquid media and are too large and variable in size to be suitable for quantitative inoculations of experimental media. A stirred- culture technique yielded uniformly small viable colonies of The microcolonies obtained still sedimented rapidly but could be kept in even suspension by bubbling nitrogen through the suspension. Such suspensions gave uniform inocula from a graduated Pasteur pipette as judged by total-nitrogen determinations and showed the growth characteristics of grown in static liquid media.

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/content/journal/micro/10.1099/00221287-23-2-261
1960-10-01
2021-10-25
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