1887

Abstract

Summary: The time course of lysis of protoplasts of was studied by spectrophotometric methods. The lytic agents used were sodium dodecylsulphate and a range of cationic detergents which each possessed a C alkyl chain and comprised the amine hydrochloride, the trimethylammonium bromide, the pyri-dinium bromide and the quinolinium bromide. Also the inhibition of lysis by uranyl ions and the action of detergents on isolated protoplast membranes were investigated. It is concluded that the cationic detergents act on the complex phosphatidic acid lipid component of the protoplast membrane and that lysis results from secondary osmotic effects. Access to the reactive site by agents of similar hydrophilic-hydrophobic balance is controlled by steric factors. Anionic detergents, however, cause direct disintegration of the protoplast, membrane independent of osmotic stresses, probably by interaction with the membrane protein moiety; a concurrent interaction with lipid, possibly through a lipoprotein complex, is not definitely excluded.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-23-1-19
1960-08-01
2024-12-08
Loading full text...

Full text loading...

/deliver/fulltext/micro/23/1/mic-23-1-19.html?itemId=/content/journal/micro/10.1099/00221287-23-1-19&mimeType=html&fmt=ahah

References

  1. Dawson I.M., Lominski I., Stern H. 1953; An electron-microscope study of the action of cetyl-trimethylammonium bromide on Staphylococcus aureus. . J. Path. Bact 66:513
    [Google Scholar]
  2. Few A.V., Gilby A.R., Ottewill R.H., Parreira H.C. 1958; Dodecylquino-linium bromide. J. chem. Soc p. 1712
    [Google Scholar]
  3. Few A.V., Gilby A.R., Seaman G.V.F. 1960; An electrophoretic study on structural components of Micrococcus lysodeifcticus. . Biochim. biophys. Acta 38:130
    [Google Scholar]
  4. Few A.V., Ottewill R.H., Parreira H.C. 1955; The interaction between bovine plasma albumin and dodecyltrimethylammonium bromide. Biochim. biophys. Acta 18:136
    [Google Scholar]
  5. Gilby A.R., Few A.V. 1957a; Reactivity of ionic detergents with Micrococcus lysodeikticus. . Nature; Lond.: 179422
    [Google Scholar]
  6. Gilby A.R., Few A.V. 1957b; Surface chemical studies on the protoplast membrane of Micrococcus lysodeikticus. . Proc. 2nd int. Congr. Surface Activity 4:262
    [Google Scholar]
  7. Gilby A.R., Few A.V., Mcquillen K. 1958; The chemical composition of the protoplast membrane of Micrococcus lysodeikticus. . Biochim. biophys. Acta 29:21
    [Google Scholar]
  8. Love L.H. 1950; The haemolysis of human erythrocytes by sodium dodecyl sulphate. J. cell. comp. Physiol 36:133
    [Google Scholar]
  9. Massart L. 1952; Substances chimothérapeutiques cationiques et compétitions entre cations. Bull. Soc. Chim. biol., Paris, 34:1145
    [Google Scholar]
  10. Newton B.A. 1954; Site of action of polymyxin on Pseudomonas aeruginosa: Antagonism by cations. J. gen. Microbiol 10:491
    [Google Scholar]
  11. Pethica B.A. 1958; Lysis by physical and chemical methods. J. gen. Microbiol 18:473
    [Google Scholar]
  12. Rideal E.K., Taylor F.H. 1957; On haemolysis by anionic detergents. Proc. roy. Soc B 146:225
    [Google Scholar]
  13. Salton M.R.J. 1957; The action of lytic agents on the surface structures of the bacterial cell. Proc. 2nd int. Congr.Surface Activity 4:245
    [Google Scholar]
  14. Tomcsik J., Guex-Holzer S. 1952; Änderung der Struktur der Bakterienzelle im Verlauf der Lysozym-Einwirkung. Schweiz. Z. allg. Path 15:518
    [Google Scholar]
  15. Weibull C. 1953; The isolation of protoplasts from Bacillus megaterium by controlled treatment with lysozyme. J. Bact 66:688
    [Google Scholar]
/content/journal/micro/10.1099/00221287-23-1-19
Loading
/content/journal/micro/10.1099/00221287-23-1-19
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error