1887

Abstract

It was shown by MacLeod and his co-workers that among bacteria only the true marine forms have a specific sodium requirement. These all require this ion in concentrations of the order of a few tenths of 1 %. The non-marine bacteria studied by MacLeod were distinguished from marine forms by their lack of any requirement for sodium (MacLeod, Onofrey & Norris, 1954; MacLeod & Onofrey, 1956). In view of these facts, the following observation, which I made in the course of studying the amino acid nutrition of , seemed significant. This bacterium can grow very well in media of ordinary ionic strength and thus is not obviously marine. I observed that it did not grow in a medium in which an artificial mixture of amino acids had replaced an acid hydrolysate of casein. Since almost 40 % of the hydrolysate used was sodium chloride, I added a little NaCl to the medium containing the artificial mixture of amino acids. I then found that the growth was as rapid as in the medium made with casein hydrolysate. It was easily shown that a very much simpler mixture of amino acids permitted rapid growth in the presence of as little as 100 g. NaCl/ml. This requirement for a low concentration of sodium seemed to set this organism apart both from marine and from terrestrial bacteria and thus to merit a more detailed analysis, the first results of which are reported here.

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1960-06-01
2021-10-22
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