1887

Abstract

Summary: Excised soybean root nodules exposed to an atmosphere containing an excess of N incorporated the label first into the centrifugal fraction containing the intracellular membrane envelopes and small amounts of bacteroid cell walls. The N concentration then increased in the soluble portion of the nodules. The bacteroid fraction was not labelled after 2 hr. exposure of the nodules to N. In ageing nodules incorporation of N into the soluble fraction declined before incorporation into the membrane fraction. The inhibitory effects of CO, NO and H on N incorporation in the various fractions were studied; the results suggested differential inhibition. The membrane fraction contained 8·5 % (w/v) total N, 37 % (w/w) lipid, had a negligible O uptake in the presence of substrates and had an absorption spectrum suggestive of the presence of porphyrin compounds. Further fractionation of the membrane fraction indicated that the N was associated with the lighter particles and was only partially soluble in 3-HCl. The possibility that the membrane fraction contained the site of primary N activation is discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-22-3-671
1960-06-01
2021-08-04
Loading full text...

Full text loading...

/deliver/fulltext/micro/22/3/mic-22-3-671.html?itemId=/content/journal/micro/10.1099/00221287-22-3-671&mimeType=html&fmt=ahah

References

  1. Aprison M.H., Burris R.H. 1952; Time course of fixation of N2 by excised soybean nodules. Science 115:264
    [Google Scholar]
  2. Aprison M.H., Magee W.E., Burris R.H. 1954; Nitrogen fixation by excised nodules. J. biol. Ckem 208:29
    [Google Scholar]
  3. Bergersen F.J. 1958; The bacterial component of soybean root nodules; changes in respiratory activity, cell dry weight and nucleic acid content with increasing nodule age. J. gen. Microbiol 19:312
    [Google Scholar]
  4. Bergersen F.J., Briggs M.J. 1958; Studies on the bacterial component of soybean root nodules; cytology and organization in the host tissue. J. gen. Microbiol 19:482
    [Google Scholar]
  5. Klüver H. 1948; On a possible use of the root nodules of leguminous plants for research in neurology and psychiatry (preliminary report on a free porphyrin-hemoglobin system). J. Psychol 25:331
    [Google Scholar]
  6. Lemberg R., Legge J.W. 1949 Haematin compounds and bile pigments p. 74 New York: Interscience Publishers Inc;
    [Google Scholar]
  7. Mcknight T. 1949; Efficiency of isolates of Rhizobium of the cowpea group, with proposed additions to this group. Qd J. agric. Sd 6:61
    [Google Scholar]
  8. Magee W.E., Burris R.H. 1954; Fixation of 15N2 by excised nodules. Plant physiol 29:199
    [Google Scholar]
  9. Turchin F.V. 1957; Scientific and Technical News. Soviet J. atomic Energy 3:940
    [Google Scholar]
  10. Virtanen A.L., Moisio T., Burris R.H. 1955; Fixation of nitrogen by nodules excised from darkened and illuminated plants. Acta chem. scand 9:184
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-22-3-671
Loading
/content/journal/micro/10.1099/00221287-22-3-671
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error