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Summary: The effects of several dyes on the growth on solid culture media of several strains of Azotobacter chroococcum, A. vinelandii, A. agile and A. beijerinckii were studied. Some of the dyes inhibited the growth of one or more of the Azotobacter spp. tested. From our results, we conclude that A. chroococcum, A. vinelandii, A. agile and A. beijerinckii are different species, well characterized by their behaviour to the dyes. We propose a scheme, based on the action of 1/25,000 (w/v) Pyronine and 1/50,000 (w/v) Diamond fuchsin which differentiates the four Azotobacter species quickly and easily.
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