1887

Abstract

SUMMARY: A strictly anaerobic mesophilic species of cellulose-decomposing bacteria was isolated from soil by a new technique. Cultures were made in screw-capped bottles with media containing finely divided cellulose. The method and apparatus used for filling these bottles with reduced media and nitrogen is described. Flocculation of cellulose particles in agar media was prevented by incorporating a low concentration of sodium carboxymethyl cellulose. Cellulolytic colonies in cellulose agar media were of two types, punctiform and spreading. The isolate derived from a punctiform colony digested cellulose with the formation of formic, acetic and malic acids, carbon dioxide and hydrogen. Essential growth factors were provided by yeast and soil extracts. Surface colonies on yeast peptone cellobiose agar were differentiated into convex entire central zones and thin transparent irregular margins. In deep culture with the same medium the isolate grew either as discrete lenticular colonies or spread rapidly throughout the agar. These growth forms were unstable, the type appearing in any one culture being unpredictable. In many respects the isolate resembles Hungate, and is probably a strain of this species. It differs from , however, in producing little or no cellobiose but abundant glucose from cellulose in liquid culture.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-22-2-539
1960-04-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/22/2/mic-22-2-539.html?itemId=/content/journal/micro/10.1099/00221287-22-2-539&mimeType=html&fmt=ahah

References

  1. Barker H. A. 1956 Bacterial Fermentations New York: John Wiley and Sons Inc; London: Chapman and Hall Ltd
    [Google Scholar]
  2. Barker H. A., Taha S. M. 1942; Clostridium kluyveri, an organism concerned in the formation of caproic acid from ethyl alcohol. J. Bact 43:347
    [Google Scholar]
  3. Clausen P. 1931; Studien über anaerobe Zellulosebazillen unter besonderer Berücksichtigung der Züchtungstechnik. Zbl. Bakt. (2. Abt.) 84:20
    [Google Scholar]
  4. Cowles P. B., Rettger L. F. 1931; Isolation and study of an apparently widespread cellulose-fermenting anaerobe, Cl. cellulosolvem (n.sp.?). J. Bact 21:167
    [Google Scholar]
  5. Elsden S. R. 1946; The application of the silica gel partition chromatogram to the estimation of volatile fatty acids. Biochem. J 40:252
    [Google Scholar]
  6. Fred E. B., Waksman S. A. 1928 Laboratory Manual of General Microbiology New York: McGraw-Hill Book Co. Inc;
    [Google Scholar]
  7. Hungate R. E. 1944; Studies on cellulose fermentation. I. The culture and physiology of an anaerobic cellulose-digesting bacterium. J. Bact 48:499
    [Google Scholar]
  8. Hungate R. E. 1947; Studies on cellulose fermentation. III. The culture and isolation of cellulose-decomposing bacteria from the rumen of cattle. J. Bact 53:631
    [Google Scholar]
  9. Hungate R. E. 1950; The anaerobic mesophilic cellulolytic bacteria. Bact. Rev 14:1
    [Google Scholar]
  10. Khouvine Y. 1923; Digestion de la cellulose par la flore intestinale de 1’homme. B. cellulose dissolvens, n.sp. Ann. Inst. Pasteur 37:711
    [Google Scholar]
  11. Lanigan G. W. 1951; The bacterial flora of Australian flax retting. Aust. J. Sci. Res. Ser B 4:461
    [Google Scholar]
  12. Miles A. A., Hayward N. J. 1943; Inhibition of Proteus in cultures from wounds. Lancet ii:116
    [Google Scholar]
  13. Omelianski W. 1902; Über die Gärung der Cellulose. Zbl. Bakt 2. Abt. Orig 8:225
    [Google Scholar]
  14. Reid R. L., Lederer M. 1951; Separation and estimation of saturated C2–C7 fatty acids by paper partition chromatography. Biochem. J 50:60
    [Google Scholar]
  15. Siu R. G. H. 1951 Microbial Decomposition of Cellulose New York: Reinhold Publishing Corp;
    [Google Scholar]
  16. Spray R. S. 1936; Semisolid media for cultivation and identification of the sporulating anaerobes. J. Bact 32:135
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-22-2-539
Loading
/content/journal/micro/10.1099/00221287-22-2-539
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error