Steroid Growth Requirements and Steroid Growth Inhibitors of Mycoplasma Free

Abstract

SUMMARY: strain A did not grow significantly and sometimes died in a peptone + yeast extract (PY) medium; good growth was obtained when 1 % serum or 1 % bovine plasma albumin was added. The addition of cholesterol and some other steroids (at 20g./ml.) to the basal PY medium gave some growth although less than that given by adding serum or albumin. Certain other steroids (e.g. Δ and Δ -cholesten-3-one) were potent inhibitors of cholesterol-promoted growth at one- tenth the cholesterol concentration. One of these steroids at 2g./ml. inhibited the growth which was obtained in PY + albumin medium; this inhibition was annulled by 200g. cholesterol/ml. Two of the inhibitory steroids at 300g./ml. inhibited the growth of strains A and B, var. and in a rich serum-containing medium. The inhibitory effects of one of these steroids was greatly diminished when it was added after growth had begun.

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/content/journal/micro/10.1099/00221287-22-2-483
1960-04-01
2024-03-29
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