1887

Abstract

SUMMARY: Determination has been made by the technique of abortive transduction of the functional relationships of over 200 histidine mutant alleles. The results are compared with those obtained by genetic recombination Hartman, Loper & Šerman, 1960 and enzyme studies Ames, Garry & Herzenberg, 1960. The results indicate that abortive transduction tests can be used to position rapidly sites of mutation on a chromosome map within the region of a single gene. The tests for genetic complementation reveal that some genes behave as single functional units, while other genes are composed of two, and some of four, functional subunits (complementation units). The existence of mutants with overlapping, intra-genic non-complementing patterns may allow crude mapping, solely by complementation, of some mutational sites within certain single genes. In gene , the ordering of mutational sites by this technique so far conforms with that obtained by the more classic methods of map construction based on inferences derived from recombination analyses. Data are presented bearing on the individuality of the gene and the effect of mutation thereon. It is concluded that some genetic damages, localized within single genes, can secondarily affect the expression of adjacent gene(s) in addition to their primary effect upon the gene in which they are located (position effect).

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/content/journal/micro/10.1099/00221287-22-2-354
1960-04-01
2022-05-19
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References

  1. Adams M. H. 1959 Bacteriophages New York: Interscience Publishers, Inc;
    [Google Scholar]
  2. Ames B. N., Garry B., Herzenberg L. 1960; The genetic control of the enzymes of histidine biosynthesis in Salmonella typhimurium . J. gen. Microbiol 22:369
    [Google Scholar]
  3. Beadle G. W., Coonradt V. L. 1944; Heterocaryosis in Neurosporacrassa . Genetics 29:291
    [Google Scholar]
  4. Benzer S. 1955; Fine structure of a genetic region in bacteriophage. Proc. nat. Acad. Sci., Wash 41:344
    [Google Scholar]
  5. Carlson E. A. 1959; Comparative genetics of complex loci. Quart. Rev. Biol 34:33
    [Google Scholar]
  6. Chovnick A., Fox A. S. 1953; Immunogenetic studies of pseudoallelism in Drosophila melanogaster. I. Antigenic effects of the lozenge pseudoalleles. Proc. nat. Acad. Sci., Wash 39:1035
    [Google Scholar]
  7. Demerec M., Hartman P. E. 1959; Complex loci in microorganisms. Annu. Rev. Microbiol 13:377
    [Google Scholar]
  8. Demerec M., Ozeki H. 1959; Tests for allelism among auxotrophs of Salmonella typhimurium . Genetics 44:269
    [Google Scholar]
  9. Frieden C. 1958; The dissociation of glutamic dehydrogenase by reduced diphosphopyridine nucleotide (DPNH). Biochim. biophys. Acta 27:431
    [Google Scholar]
  10. Giles N. H. 1958; Mutations at specific loci in Neurospora . Proc. 10th int. Congr. Genetics, Montreal 1:261
    [Google Scholar]
  11. Gots J. S., Coon R. G., Hild D. H. 1959; Cistronic control of biosynthetic leakage in purine auxotrophs of Salmonella typhimurium . Bact. Proc p. 35
    [Google Scholar]
  12. Hartman P. E. 1957; Transduction: a comparative review. In A Symposium on The Chemical Basis of Heredity McElroy W. D., Glass B. ed pp. 408–62 Baltimore: The Johns Hopkins Press;
    [Google Scholar]
  13. Hartman P. E., Goodgal S. H. 1959; Bacterial genetics (with particular reference to genetic transfer). Annu. Rev. Microbiol 13:465
    [Google Scholar]
  14. Hartman P. E., Hartman Z. 1957; Histidine-requiring mutants of Salmonella typhimurium: Complete and abortive transduction. Heredity 11:286
    [Google Scholar]
  15. Hartman P. E., Loper J. C., Šerman D. 1960; Fine structure mapping by complete transduction between histidine-requiring Salmonella mutants. J. gen. Microbiol 22:323
    [Google Scholar]
  16. Lederberg J. 1956; Linear inheritance in transductional clones. Genetics 41:845
    [Google Scholar]
  17. Lewis E. B. 1950; The phenomenon of position effect. Advanc. Genet 3:73
    [Google Scholar]
  18. Markert C. L. 1958; Chemical concepts of cellular differentiation. In Chemical Basis of Development McElroy W. D., Glass B. ed pp. 3–16 Baltimore: The Johns Hopkins Press;
    [Google Scholar]
  19. Muller H. J., Prokofyeva A. A. 1935; The individual gene in relation to the chromomere and the chromosome. Proc. nat. Acad. Sci., Wash 21:16
    [Google Scholar]
  20. Ozeki H. 1956; Abortive transduction in purine-requiring mutants of Salmonella typhimurium . Genetic Studies voith Bacteria. Publ. Carneg. Instn612 p. 97
    [Google Scholar]
  21. Stocker B. A. D. 1956; Abortive transduction of motility in Salmonella . J. gen. Microbiol 15:575
    [Google Scholar]
  22. Stocker B. A. D., Zinder N. D., Lederberg J. 1953; Transduction of flagellar characteristics in Salmonella . J. gen. Microbiol 9:410
    [Google Scholar]
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