1887

Abstract

SUMMARY: Evidence is presented which suggests that after a period of adsorption of 30 min. the herpes virus which could not be removed from HeLa cells by repeated washing existed in four fractions: Fraction A, about 63 % which became non-infective and insusceptible to antiserum over the next 2 hr.; fraction B, about 16 % which remained susceptible to antiserum and infective for up to 6 hr. after adsorption; fraction C, about 2 %, which remained infective and insusceptible to antiserum for at least 6 hr. after adsorption; fraction D, about 19 %, which became non-infective and insusceptible to antiserum during the adsorption period. Replication occurred from fractions A and D. Inactivation and the development of insusceptibility to antiserum occurred very close together in time, possibly simultaneously. Incubation of infected cells in the presence of antiserum lowered the yield of virus obtained after 48 hr. incubation; this could not be explained simply by prevention of secondary infection through the medium.

Erratum

An erratum has been published for this content:
CORRIGENDA
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-22-1-57
1960-02-01
2022-10-02
Loading full text...

Full text loading...

/deliver/fulltext/micro/22/1/mic-22-1-57.html?itemId=/content/journal/micro/10.1099/00221287-22-1-57&mimeType=html&fmt=ahah

References

  1. Ackermann W.W., Ishida N., Maassab H.F. 1955; Growth characteristics of influenza virus concerning the binding of virus by host cells. J. exp. Med 102:545
    [Google Scholar]
  2. Bedson S.P., Gostling J.V.T. 1958; Further observations on the mode of multiplication of herpes virus. Brit. J. exp. Path 39:502
    [Google Scholar]
  3. Farnham A.E. 1958; The formation of microscopic plaques by herpes simplex virus in HeLa cells. Virology 6:817
    [Google Scholar]
  4. Ishida N., Ackermann W.W. 1956; Growth characteristics of influenza virus. Properties of the initial cell-virus complex. J. exp. Med 104:501
    [Google Scholar]
  5. Stoker M.G.P., Ross R.W. 1958; Quantitative studies on the growth of herpes virus in HeLa cells. J. gen. microbiol 19:250
    [Google Scholar]
  6. Watkins J.F. 1960; The adsorption, penetration and eclipse of herpes simplex virus in chorio-allantoic membrane ectoderm. J. gen. microbiol 22:40
    [Google Scholar]
  7. Wildy P., Stoker M.G.P., Ross R.W. 1959; Release of herpes virus from solitary HeLa cells. J. gen. microbiol 20:105
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-22-1-57
Loading
/content/journal/micro/10.1099/00221287-22-1-57
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error