SUMMARY: The kinetics of the early stages of infection by herpes virus were studied in the chorio-allantoic membrane (CAM). Adsorption followed approximately first-order kinetics and was temperature-dependent. Adsorbed virus was not removed from the CAM by repeated washing after various adsorption periods up to 60 min. Forty to 70% of the adsorbed firmly bound virus was neutralized by anti-herpes serum at the end of the adsorption period, and became gradually insusceptible to antiserum over the next three hours. Only about 0·1% of this adsorbed virus was recovered by freezing and grinding infected CAM's at the end of adsorption, although the recovery procedures themselves did not inactivate the virus. Because of the failure to demonstrate any other reason for the loss of over 99% of adsorbed virus it is suggested that virus had lost its infectivity very rapidly upon adsorption at a time when a considerable part of it was susceptible to antiserum. Growth of new virus occurred from the non-recoverable virus.


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