1887

Abstract

SUMMARY: The proteolytic activity of the whole rumen fluid of sheep does not depend on the diet of the animals. The optimum pH value for proteolysis lies between 6 and 7, with an irregular maximum at about pH 6·5. All fractions of rumen contents (protozoa, large bacteria and small bacteria) show activity. Proteolytic activity can be demonstrated in the supernatant liquid after disruption of the microorganisms. The proteolytic activity of the whole rumen fluid was enhanced by cysteine at 0·06 and 0·006 % (w/v) unless the rumen fluid had been collected and processed under highly anaerobic conditions. Potassium cyanide and ascorbic acid were also slightly stimulatory, but sodium thiosulphate, thioglycollic acid, sodium sulphide and Fe, Ca and Mg++ ions had no effect.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-22-1-272
1960-02-01
2021-10-22
Loading full text...

Full text loading...

/deliver/fulltext/micro/22/1/mic-22-1-272.html?itemId=/content/journal/micro/10.1099/00221287-22-1-272&mimeType=html&fmt=ahah

References

  1. Annison E. F. 1956; Nitrogen metabolism in the sheep. Biochem. J 64:705
    [Google Scholar]
  2. Anson M. L. 1988; The estimation of pepsin, trypsin, papain and cathepsin with haemoglobin. J. gen. Physiol 22:79
    [Google Scholar]
  3. Appleby J. C. 1955; The isolation and classification of proteolytic bacteria from the rumen of the sheep. J. gen. Microbiol 12:526
    [Google Scholar]
  4. Blackburn T. H., Hobson P. N. 1958; Some studies on the isolation of proteolytic bacteria from the rumen. Proc. Nutr. Soc 17:xxv
    [Google Scholar]
  5. Blackburn T. H., Hobson P. N. 1960; Breakdown of protein and proteolytic activity in the sheep rumen at different times after feeding. J. gen. Microbiol 22:290
    [Google Scholar]
  6. Eadie J. M., Oxford A. E. 1955; Factors involved in the production of a novel kind of derangement of storage mechanism in living holotrich ciliate protozoa from sheep rumen. J. gen. Microbiol 12:298
    [Google Scholar]
  7. Folin C., Ciocalteu V. 1927; Tyrosine and tryptophane determinations in proteins. J. biol. Chem 73:627
    [Google Scholar]
  8. Hobson P. N. 1959; Nitrogen metabolism in the rumen with information on the organisms concerned. Proc. Conference on Radioactive Isotopes in Agriculture Oklahoma: In the Press
    [Google Scholar]
  9. Hunt W. G. 1957 A study of proteolytic factors of rumen micro-organisms Ph.D. thesis Ohio State University:
    [Google Scholar]
  10. Hunt W. G., Moore R. O. 1958; The proteolytic system of a Gram-negative rod isolated from the bovine rumen. Appl. Microbiol 6:36
    [Google Scholar]
  11. Layne E. 1957; Spectrophotometric and turbidimetric methods for measuring proteins. Methods in Enzymology 8 New York: Academic Press Inc;
    [Google Scholar]
  12. McDonald I. W. 1954; The extent of conversion of food protein to microbial protein in the sheep. Biochem. J 56:120
    [Google Scholar]
  13. McDonald I. W. 1957; The conversion of casein into microbial proteins in the sheep. Biochem. J 67:400
    [Google Scholar]
  14. Mickle H. 1948; Tissue disintegrator. J. roy. micr. Soc 68:10
    [Google Scholar]
  15. Moir R. J., Masson M. J. 1952; An illustrated scheme for the microscopic identification of the rumen micro-organisms of sheep. J. Path. Bact 64:843
    [Google Scholar]
  16. Oxford A. E. 1955; The rumen ciliate protozoa. Exp. Parasitol 4:569
    [Google Scholar]
  17. Tracey M. V. 1948; Leaf protease of tobacco and other plants. Biochem. J 42:281
    [Google Scholar]
  18. Warner A. C. I. 1956; Proteolysis by rumen micro-organisms. J. gen. Microbiol 14:788
    [Google Scholar]
  19. Weller R. A., Gray F. V., Pilgrim A. F. 1958; The conversion of plant nitrogen to microbial nitrogen in the rumen of the sheep. Brit. J. Nutr 12:421
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-22-1-272
Loading
/content/journal/micro/10.1099/00221287-22-1-272
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error