@article{mbs:/content/journal/micro/10.1099/00221287-22-1-184, author = "Nicholas, D. J. D. and Mabey, G. L.", title = "Some Properties of Glutamic Dehydroǵenase from Neurospora crassa", journal= "Microbiology", year = "1960", volume = "22", number = "1", pages = "184-190", doi = "https://doi.org/10.1099/00221287-22-1-184", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-22-1-184", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY: A glutamic dehydrogenase from Neurospora requiring either DPNH or TPNH for activity has a pH optimum of 8·6 and is inhibited by a number of chelating agents. The specific activity of the enzyme was high during early growth, but it fell sharply from the 2nd to the 5th day after inoculation. A deficiency of Zn decreased enzyme activity and addition of the metal in vivo to Zn-deficient mycelial felts restored the enzyme to normal values after a few days of incubation. The enzyme was unchanged when pyridoxine or riboflavin was limiting in the growth of mutants which require these vitamins. With a purified enzyme the Km values in mole/l. were as follows: α-ketoglutarate 0·75 × 107#x2212;2; TPNH, 2 × l0−4; and DPNH, 2·3 × 10−4.", }