1887

Abstract

SUMMARY: The wild-type strain of 2.4.1 does not utilize exogenously supplied gluconate and grows poorly with glucose, fructose or mannose as substrates, accumulating aldonic acids and 2-keto-3-deoxy gluconic acid in media containing these sugars. Mutants which are capable of growing well with glucose without the accumulation of acids acquire the enzyme ‘phosphoglu-conic acid dehydrase’ and oxidize hexoses mainly via the pathway described previously in . The Embden-Meyerhof pathway is constitutive in the wild-type and mutant strains of but appears to have a limited function as a result of very low aldolase activity. Phosphogluconic acid dehydrogenase is either absent or too low in activity to permit the utilization of the pentose-phosphate oxidative pathway.

A new type of particulate ‘aldose dehydrogenase system’, constitutive in all strains is involved in the oxidation of glucose and mannose to the corresponding aldonic acid lactones. Gluconic and mannonic acids are both converted to 2-keto- 3-deoxy acid through the action of two distinct dehydrating enzymes. A mutation leading to the utilization of gluconic acid is associated with the acquisition of a high level of ‘2-keto-3-deoxy gluconic acid kinase’, but not of phosphogluconic acid kinase. A permeability factor may also be involved in the adaptation of gluconate.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-22-1-167
1960-02-01
2024-11-04
Loading full text...

Full text loading...

/deliver/fulltext/micro/22/1/mic-22-1-167.html?itemId=/content/journal/micro/10.1099/00221287-22-1-167&mimeType=html&fmt=ahah

References

  1. Ashwell G., Wahba A.J., Hickmam J. 1958; A new pathway of uronic acid metabolism. Biochim. biophys.Acta 30:186
    [Google Scholar]
  2. Block RJ., Dubrum E.I., Zweig G. 1958 A Manual of Paper Chromatography and Electrophoresis p. 207 New York: Academic Press Inc;
    [Google Scholar]
  3. Cohen-Bazire G., Sistrom W.R., Stanier R.Y. 1957; Kinetic studies of pigment synthesis by non-sulphur purple bacteria. J. cell. comp. Physiol 49:25
    [Google Scholar]
  4. Cynkin M.A., Ashwell G. 1959; Phosphoryiation of 2-keto-3-deoxygluconic acid by extracts of Escherichia Coli. . Bact. Proc p. 108
    [Google Scholar]
  5. De Ley J., Doudoroff M. 1957; The metabolism of D-galactose in Pseudomonas saccharophila. . J. biol. Chem 227:745
    [Google Scholar]
  6. Bntner N. 1958; On the pathway of carbohydrate metabolism in Entamoeba histolytica. . J. Parasit 44:688
    [Google Scholar]
  7. Entner N., Doudoroff M. 1952; Glucose and gluconic acid oxidation by Pseudomonas saccharophila . J. biol. Chem 196:858
    [Google Scholar]
  8. Folin O., Malmros H. 1929; An improved form of Folin’s micro method for blood sugar determinations. J. biol. Chem 83:115
    [Google Scholar]
  9. Friedmann T.E., Haugen G.E. 1943; Pyruvic acid. II. The determination of Keto acids in blood and urine. J. biol. Chem 147:415
    [Google Scholar]
  10. Hanes C.S., Ishbrwood F.A. 1949; Separation of the phosphoric esters on the Biter paper chromatogram. Nature; London: 641107
    [Google Scholar]
  11. Hestrin S. 1949; The reaction of acetylcholine and other carboxylic acid derivatives with hydroxylamine, and its analytical application. J. biol. Chem 180:249
    [Google Scholar]
  12. Hochster R.M., Katznelson H. 1958; On the mechanism of glucose-6- phosphate oxidation in cell-free extracts of Xanthomonas phaseoli (XP8). Canad. J. Biochem. Physiol 36:669
    [Google Scholar]
  13. Kilgore W.W., Rush J.D., Starr M.P. 1959; bacterial oxidation of uronic acids. bact Proc p. 103
    [Google Scholar]
  14. Kovachevicn R., Wood W.A. 1955a; Carbohydrate metabolism by Pseudomonas fluorescens. III. Purification and properties of 6-phosphogluconate dehydrase. J. biol. Chem 213:745
    [Google Scholar]
  15. Kovachevich R., Wood W.A. 1955b; Carbohydrate metabolism by Pseudomonas fluorescens.. IV. Purification and properties of 2-keto-3-deoxy-6-phosphogluconate aldose. J. biol. Chem 213:757
    [Google Scholar]
  16. Lanning M.C., Cohen S.S. 1951; The detection and estimation of 2-keto- hexonic acids. J. biol. Chem 189:109
    [Google Scholar]
  17. Lowry O.H., Rosebrugh N.J., Farr A.L., Randall R.V. 1951; Protein measurement with the Folin phenol reagent. J. biol. Chem 193:265
    [Google Scholar]
  18. Macgee J., Doudoroff M. 1954; A new phosphorylated intermediate in glucose oxidation. J. biol. Chem 210:617
    [Google Scholar]
  19. Mcllwaine H. 1948; Preparation of cell free bacterial extracts with powdered alumina. J. gen. microbiol 2:288
    [Google Scholar]
  20. Palleroni N., Doudoroff M. 1956; Preparation and properties of d-rham- nulose (6-deoxy-d-fructose) and glucosyl rhamnuloside. J. biol. Chem 219:957
    [Google Scholar]
  21. Palleroni N., Doudoroff M. 1957; Metabolism of carbohydrates by Pseudomonas saccharophila, III. Oxidation of d-arabinose. J. bact 74:180
    [Google Scholar]
  22. Roe J.H. 1934; A colorimetric method for the determination of fructose in blood and urine. J. biol. Chem 10:7–15
    [Google Scholar]
  23. Schales O., Schales S.S. 1945; A simple method for the determination of glucose in blood. Arch. Biochem 8:285
    [Google Scholar]
  24. Taylor J.F. 1955; Aldolase from muscle. Methods in Enzymology Colowick S.P., Kaplan N.O. Ed. 1 p. 310 New York: Academic Press Inc;
    [Google Scholar]
  25. Treveleyan W.E., Procter D.P., Harrison J.S. 1950; Detection of sugar on paper chromatograms. Nature; Lond.: 166444
    [Google Scholar]
  26. Van Niel C.B. 1944; The culture, general physiology, morphology and classification of the non-sulfur purple and brown bacteria. Bact. Rev 8:1
    [Google Scholar]
  27. Weimberg R. 1959; l-2-Keto-4,5-dihydroxy valeric acid: an intermediate in the oxidation of l-arabinose by Pseudomonas saccharophila . J. biol. Chem 234:727
    [Google Scholar]
/content/journal/micro/10.1099/00221287-22-1-167
Loading
/content/journal/micro/10.1099/00221287-22-1-167
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error