1887

Abstract

SUMMARY: strain K112 was infected with λ phage (multiplicity of input 20 particles/cell) in 0·02 -MgSO, and 91 % infected cells gave the lysogenic response. When the infected cells were transferred to a complete amino acid medium containing C--phenylalanine and a non-utilizable inducer for -galactosidase, the net synthesis of protein and nucleic acids did not begin until after 100–120 min. During this period there was no induced synthesis of -galactosidase (though the enzyme was readily developed in uninfected cells) and there was no increase in turbidity of the culture or in viable cell count. The synthesis of protein and nucleic acids began in uninfected cells immediately they were placed in the complete medium, turbidity increased from the start and the viable count after 30 min. This suggested that during lysogenization the infecting phage temporarily halts most if not all of the synthetic activities of the cell and thus forms an environment in which the phage genome can become attached to the bacterial chromosome and establish the prophage state.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-21-3-685
1959-12-01
2021-10-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/21/3/mic-21-3-685.html?itemId=/content/journal/micro/10.1099/00221287-21-3-685&mimeType=html&fmt=ahah

References

  1. Bertani G. 1953; Lysogenic versus lytic cycle of phage multiplication. Cold Spr. Harb. Symp. quant. Biol 18:65
    [Google Scholar]
  2. Bertani G. 1958; Lysogeny. Advanc. Virus Res 5:151
    [Google Scholar]
  3. Bertani G., Nice S. J. 1954; Studies on lysogenesis. 2. The effect of temperature on the lysogenisation of Shigella dysenteriae with phage P 1. J. bact 67:202
    [Google Scholar]
  4. Boyd J. S. K. 1951; Excessive dose phenomenon in virus infection. Nature; London: 1671061
    [Google Scholar]
  5. Burton K. 1956; A study of the conditions and mechanism of the diphenyl-amine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J 62:315
    [Google Scholar]
  6. Christensen J. R. 1957; Effect of chloramphenicol on lysogenisation by temperate phage P 1. Virology 4:184
    [Google Scholar]
  7. Cohn M., Monod J. 1951; Purification et propriétés de la β-galactosidase (lactase) d’Escherichia Coli. . Biochim. biophys. Acta 7:153
    [Google Scholar]
  8. Cohn M., Monod J. 1953; Specific inhibition and induction of enzyme biosynthesis. In Adaptation in Micro-organisms Gale E. F., Davies R. Ed. Symp. Soc. gen. microbiol. 3:132
    [Google Scholar]
  9. French R. C., Siminovitch L. 1955; The action of T2 bacteriophage ghosts on Escherichia Coli B. Canad. J. microbiol 1:754
    [Google Scholar]
  10. Fry B. A. 1959; Conditions for the establishment of lysogeny in Escherichia Coli. . J. gen. microbiol 21:676
    [Google Scholar]
  11. Fry B. A., Gros F. 1958; The establishment of lysogeny in Escherichia Coli. . J. gen. microbiol 18:x
    [Google Scholar]
  12. Herriott R. M. 1951; Nucleic-acid-free T2 virus ghosts with specific biological action. J. bact 61:752
    [Google Scholar]
  13. Hershey A. D. 1955; The organisation of genetic material in bacteriophage T2 . In Mutation. Brookhaven Symp. Biol 8:6
    [Google Scholar]
  14. Hershey A. D. 1957; bacteriophages as genetic and biochemical systems. Advanc. Virus Res 4:25
    [Google Scholar]
  15. Kozloff L. M. 1953; Origin and fate of bacteriophage material. Cold Spr. Harb. Symp. quant. Biol 17:207
    [Google Scholar]
  16. Lederberg J. 1950; The β-d-galactosidase of Escherichia Coli strain K12. J. bact. 60:381
    [Google Scholar]
  17. Levine M. 1955; The production of lysogeny for virulent bacteriophage by mixed infection. Genetics 40:582
    [Google Scholar]
  18. Lieb M. 1953; The establishment of lysogenicity in Escherichia Coli. . J. bact 65:642
    [Google Scholar]
  19. Lwoff A. 1954–5; Control and interrelations of metabolic and viral diseases of bacteria. Harvey Led 50:
    [Google Scholar]
  20. Lwoff A., Kaplan A. S., Ritz E. 1954; Research on the lysogenisation of Salmonella typhi-murium. . Ann. Inst.Pasteur 86:127
    [Google Scholar]
  21. Monod J., Cohen-Bazire G., Cohn M. 1951; Sur la biosynthese de la β-galac-tosidase (lactase) chez Escherichia Coli. La specificité de I’induction. Biochim. biophys. Acta 7:585
    [Google Scholar]
  22. Schneider W. C. 1945; Phosphorus compounds in animal tissues. 1. Extraction and estimation of deoxypentose nucleic acid and of pentose nucleic acid. J. biol. Chem 161:293
    [Google Scholar]
  23. Stent G. S., Fuerst C. R. 1956; Decay of incorporated radioactive phosphorus during the development of a temperate bacteriophage. Virology 2:737
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-21-3-685
Loading
/content/journal/micro/10.1099/00221287-21-3-685
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error