1887

Abstract

SUMMARY: The structure of tobacco rattle virus was studied by examining with the electron microscope: (1) shadow-cast mounts of particles partly degraded with alkali, sodium dodecyl sulphate or phenol; (2) unshadowed mounts on thin carbon film of particles treated with solutions of lanthanum nitrate, uranyl acetate, osmium tetroxide, phosphomolybdic acid or phosphotungstic acid.

The particles were tubular, with a central hole of approximately 4 m diameter and an outside diameter which varied from 17 to 25 m according to treatment. Next to the central hole, which could be filled with lanthanum nitrate or with uranyl acetate, was a region 1–1·5 m thick, which stained heavily with osmium tetroxide, phosphomolybdic and phosphotungstic acids. The rest of the particle stained lightly with uranyl acetate, phosphomolybdic and phosphotungstic acids, and showed transverse bands 2·5 m apart. It is suggested that these bands may represent a helical structure similar to that of tobacco mosaic virus, which tobacco rattle virus resembles in many respects.

Some properties of tobacco rattle virus have already been reported (Harrison & Nixon, 1959) and we now describe the structure of the particles as revealed by electron microscopy.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-21-3-582
1959-12-01
2021-07-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/21/3/mic-21-3-582.html?itemId=/content/journal/micro/10.1099/00221287-21-3-582&mimeType=html&fmt=ahah

References

  1. Brenner S., Horne R. W. 1959; A negative staining method for high resolution electron microscopy of viruses. Biochim. biophys. Acta 34:103
    [Google Scholar]
  2. Gierer A., Schramm G. 1956; Infectivity of ribonucleic acid from tobacco mosaic virus. Nature; Lond: 177702
    [Google Scholar]
  3. Harrington W. F., Schachman H. K. 1956; Studies on the alkaline degradation of tobacco mosaic virus. I. Ultracentrifugal analysis. Arch. Biochem. Biophys 65:278
    [Google Scholar]
  4. Harrison B. D., Nixon H. L. 1959; Separation and properties of particles of tobacco rattle virus with different lengths. J. gen. Microbiol 21:569
    [Google Scholar]
  5. Hart R. G. 1955; Electron-microscopic evidence for the localization of ribonucleic acid in the particles of tobacco mosaic virus. Proc. nai. Acad. Sci., Wash 41:261
    [Google Scholar]
  6. Holmes K. C., Franklin R. E. 1958; The radial density distribution in some strains of tobacco mosaic virus. Virology 6:328
    [Google Scholar]
  7. Huxley H. E. 1957; Some observations on the structure of tobacco mosaic virus. Proc. int. Conf. Electron Microscopy, Stockholm 1956 p. 260
    [Google Scholar]
  8. Nixon H. L., Fisher H. L. 1958; An improved spray droplet technique for quantitative electron microscopy. Brit. J. appl. Phys 9:68
    [Google Scholar]
  9. Schramm G. 1947; Über die Spaltung des Tabakmosaikvirus und die Wieder- vereinigung der Spaltstücke zu höhermolekularen .Proteinen. I. Die Spaltungsreaktion. Z. Naturf 2b:112
    [Google Scholar]
  10. Sreenivasaya M., Pirie N. W. 1938; The disintegration of tobacco mosaic virus preparations with sodium dodecyl sulphate. Biochem. J 32:1707
    [Google Scholar]
  11. Williams R. C. 1952; High-resolution electron microscopy of the particles of tobacco mosaic virus. Biochim. biophys. Acta 8:227
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-21-3-582
Loading
/content/journal/micro/10.1099/00221287-21-3-582
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error