1887

Abstract

SUMMARY: A simple medium is described which allows adequate growth of with the production of only trace amounts of neuraminidase. When added to this medium to give a final concentration of 600 , sialyl lactose or sialyl-- acetylgalactosamine, two oligosaccharides which contain ketosidically bound -acetylneuraminic acid and which are substrates of neuraminidase, caused at least a 100-fold increase in the amount of neuraminidase produced during growth of the organisms. -Acetylneuraminic acid was also found to stimulate neuraminidase production. Galactose and -acetylgalactosamine had no effect. -Acetylneura- minic acid was metabolized by as indicated by the disappearance from such enriched medium of substances giving the direct Ehrlich reaction. Evidence is presented which suggests that neuraminidase is an extracellular enzyme.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-21-3-550
1959-12-01
2021-07-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/21/3/mic-21-3-550.html?itemId=/content/journal/micro/10.1099/00221287-21-3-550&mimeType=html&fmt=ahah

References

  1. Ada G. L., French E. L. 1950; Purification of the receptor destroying enzyme of V. cholerae . Aust. J. Sci 13:82
    [Google Scholar]
  2. Ada G. L., French E. L. 1957; Stimulation of the production of the receptor destroying enzyme (RDE) of V. cholerae by neuraminic acid derivatives. Aust. J. Sci 19:227
    [Google Scholar]
  3. Bacon J. S. D., Edelman J. 1951; The carbohydrates of the Jerusalem artichoke and other Compositae. Biochem. J 48:114
    [Google Scholar]
  4. Breuer R. 1898; Über das freie Chitosamin. Ber. dtsch. ehern. Ges 31:2193
    [Google Scholar]
  5. Burnet F. M., McCrea J. F., Stone J. D. 1946; Modification of human red cells by virus action; 1. The receptor gradient for virus action in human red cells. Brit. J. exp. Path 27:228
    [Google Scholar]
  6. Burnet F. M., Stone J. D. 1947; The receptor destroying enzyme of V. cholerae . Aust. J. exp. Biol. med. Sei 25:227
    [Google Scholar]
  7. Feldman W. 1958; Production of receptor destroying enzyme by Vibrio comma in synthetic medium. J. Bact 75:26
    [Google Scholar]
  8. Finkelstein R. A., Lankford C. E. 1955; Nutrient requirements of Vibrio cholerae . Bact. Proc p. 49
    [Google Scholar]
  9. Gottschalk A. 1955; 2-Carboxypyrrole : its preparation from and its precursor in mucoproteins. Biochem. J 61:298
    [Google Scholar]
  10. Gottschalk A. 1956; Neuraminic acid: the functional group of some biologically active mucoproteins. Yale J. Biol. Med 28:525
    [Google Scholar]
  11. Gottschalk A. 1957; Neuraminidase: the specific enzyme OF influenza virus and Vibrio cholerae . Biochim. biophys. Acta 23:645
    [Google Scholar]
  12. Gottschalk A., Graham E. R. B. 1958; A neuraminidase susceptible disaccharide from bovine salivary mucoprotein. Z. Naturf 13b:821
    [Google Scholar]
  13. Gottschalk A., Lind P. E. 1949; Product of interaction between influenza virus enzyme and ovomucin. Nature; Lond: 164232
    [Google Scholar]
  14. Heimer R., Mever K. 1956; Studies on sialic acid of submaxillary mucoid. Proc. nat. Acad. Sci., Wash 42:728
    [Google Scholar]
  15. Isaacs A., Edney M. 1950; Variation in laboratory stocks of influenza viruses: genetic aspects of the variations. Brit. J. exp. Path 31:209
    [Google Scholar]
  16. Kuhn R., Brossmer R. 1956; Über O-acetyl-lactaminsäure-lactose aus Kuhcolostrum und ihre Spaltbarkeit durch Influenza-virus. Ber. dtsch. chem. Ges 89:2013
    [Google Scholar]
  17. McCrea J. F. 1947; Modification of red cell agglutinability by Cl. welchii toxins. Aust. J. exp. Biol. med. Sei 25:127
    [Google Scholar]
  18. Morgan W. T. J., Elson L. A. 1934; A colorimetric method for the determination of N-acetylglucosamine and N-acetylchondrosamine. Biochem. J 28:988
    [Google Scholar]
  19. Partridge S. M. 1949; Aniline hydrogen phthallate as a spraying reagent for chromatography of sugars. Nature; Lond: 164443
    [Google Scholar]
  20. Reissig J. L., Strominger J. L., Leloir L. F. 1955; A modified colorimetric method for the estimation of N-acetylamino sugars. J. biol. Chem 217:959
    [Google Scholar]
  21. Werner I., Odin L. 1952; On the presence of sialic acid in certain glycoproteins and in gangliosides. Acta Soc. Med. upsalien 57:230
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-21-3-550
Loading
/content/journal/micro/10.1099/00221287-21-3-550
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error