1887

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Volume 21, Issue 3

Bacterial Mutation in a Stationary Phase and the Question of Cell Turnover Free

Abstract

SUMMARY: During a stationary phase induced and maintained by the exhaustion of histidine, the total number of histidineless (h−) remains constant as does the cytological appearance of the cells. If glucose is available to the starved bacteria they die at a rate of . 10 per hr., while mutations to a histidine-independent (h +) condition occur at a rate of 10 per bacterium per hr. Bacteria adapted to use lactose behave essentially the same way when it, instead of glucose, is available during starvation; but if the starved cells are not fully adapted, death does not occur or is very slow (. 10 per hr.) and the rate of mutation is 10. When no carbon source is available to the starved cells, mutations cannot be detected.

The following predictions served as tests of the hypothesis of -turnover, wherein some bacteria lyse only to be replaced at the same rate by the growth of others— the mutations are presumed to have occurred during this cryptic growth:

  1. Mixtures of h lac and h lac bacteria in lactose medium where the h lachas a selective advantage should show population shifts if cryptic growth were occurring.
  2. During the hypothesized lysis and growth the enzyme -galactosidase should, at predictable rates, be lost to the medium from adapted cultures in the absence of lactose and developed in unadapted cultures in its presence.
  3. Penicillin should kill those cells that grow to replace others, causing an accelerated death and preventing the mutations from taking place.
  4. The lysis and death might be microscopically observable on an agar surface.

    The hypothesis of turnover did not withstand any of these tests. It was concluded, therefore, that the bacteria under investigation were not dividing and that mutations —genotypic changes (Ryan, 1955 )—were taking place among them.

Reasons are given to suppose that the mutations result from errors in the replication of genetic material which is in the process of turnover within the non-dividing cells.

  • Received:
  • Published Online:
© Society for General Microbiology 1959
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1959-12-01
2023-06-09
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References

  1. Benzer S. 1953; The induced synthesis of enzymes in bacteria analyzed at the cellular level. Biochim. biophys. Acta 11:383
     [Google Scholar]
  2. Cohn M., Torriani A. M. 1953; The relationships in biosynthesis of the β-galactosidase- and PZ-proteins in Escherichia coli. Biochim. biophys. Acta 10:280
     [Google Scholar]
  3. Mandelstam J. 1957; Turnover of protein in starved bacteria and its relationship to the induced synthesis of enzyme. Nature; Lond: 1791179
     [Google Scholar]
  4. Mandelstam J. 1958; Turnover of protein in growing and non-growing populations of Escherichia coli. Biochem. J 69:110
     [Google Scholar]
  5. Markovich H. 1958; Recherches sur la mutabilité spontanée de Escherichia coli. Proc. Xth int. Congr. Genetics 2:179
     [Google Scholar]
  6. Meselson M., Stahl F. 1958; The replication of DNA in Escherichia coli. Proc. Nat. Acad. Sci., Wash 44:671
     [Google Scholar]
  7. Monod J. 1949; The growth of bacterial cultures. Annu. Rev. Microbiol 3:371
     [Google Scholar]
  8. Novick A., Wiener M. 1957; Enzyme induction as an all-or-none phenomenon. Proc. Nat. Acad. Sci., Wash 43:553
     [Google Scholar]
  9. Ryan F. J. 1955a; Spontaneous mutation in non-dividing bacteria. Genetics 40:726
     [Google Scholar]
  10. Ryan F. J. 1955b; Phenotypic (phenomic) lag in bacteria. Amer. Nat 89:159
     [Google Scholar]
  11. Ryan F. J., Kiritani K. 1959; Effect of temperature on natural mutation in bacteria. J. gen. Microbiol 20:644
     [Google Scholar]
  12. Ryan F. J., Rudner R., Nagata T., Kitani Y. 1959; Bacterial mutation and the synthesis of macromolecules. Z. VererbLehre 90:148
     [Google Scholar]
  13. Ryan F. J., Schneider L. K. 1949a; Mutations during the growth of biochemical mutants of Escherichia coli. Genetics 34:72
     [Google Scholar]
  14. Ryan F. J., Schneider L. K. 1949b; The consequences of mutation during the growth of biochemical mutants of Escherichia coli. IV. The mechanism of inhibition of histidine independent bacteria by histidineless bacteria. J. Bact 58:201
     [Google Scholar]
  15. Stubbe H. 1936; Die Erhöhung der Genmutationsrate in alterndenGonen von Antirrhinum/majus L. Biol. Zbl 56:562
     [Google Scholar]
  16. Trucco R. E., Pardee A. B. 1958; Synthesis of Escherichia coli cell walls in the presence of penicillin. J. biol. Chem 230:435
     [Google Scholar]
  17. Wainwright L. K. 1956; Spontaneous mutation in stored spores of a Streptomyces sp. J. gen. Microbiol 14:533
     [Google Scholar]
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Bacterial Mutation in a Stationary Phase and the Question of Cell Turnover
Microbiology 21, 530 (1959); https://doi.org/10.1099/00221287-21-3-530
/content/journal/micro/10.1099/00221287-21-3-530
/content/journal/micro/10.1099/00221287-21-3-530
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