1887

Abstract

SUMMARY: The nuclei and small-particle (mitochondria + microsomes) fractions, separated from 510 cultured chick embryo cells during one-step growth of vesicular stomatitis virus, were fractionated into RNA nucleotides by NaCl extraction, hydrolysis and paper electrophoresis. This allowed determination of molar and specific activity ratios of the RNA nucleotides during complementary P ‘gain’ and ‘loss’ experiments. Early exponential virus release coincided with a significant increase in uridine content of nuclear and small-particle RNA over uninfected values, and a decrease in specific activity of small-particle RNA uridylic acid in P gain experiments. Other fractions showed no change. This suggested a relatively large synthesis of an RNA different from cellular RNA and containing a high proportion of uridine at a time when virus nucleic acid synthesis was probably at its peak. This synthesis appeared to be balanced by a simultaneous breakdown of preexisting RNA.

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/content/journal/micro/10.1099/00221287-21-3-510
1959-12-01
2021-07-23
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