The Composition of the Spore Wall and the Wall of Vegetative Cells of Free

Abstract

SUMMARY: The spore wall of is composed largely of structural protein, in contrast to the peptide+amino sugar + sugar complex of the wall of vegetative cells. Alanine, glutamic acid, -diaminopimelic acid, glucosamine, muramic acid, glucose, ribitol compounds and O-ester groups are the principal constituents of the vegetative cell walls. All of the compounds characteristic of the peptide+amino sugar+sugar complex have been detected in the spore wall preparations but they account only for a minor part of the spore wall. The walls of the vegetative bacteria have a high phosphorus content and contain appreciable amounts of a ribitol phosphate polymer. Spore walls and vegetative cell walls differ also in the nature of their N-terminal amino acids; glycine is the principal N-terminal amino acid of the spore wall protein whereas alanine is the main amino acid found in the wall of vegetative cells.

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/content/journal/micro/10.1099/00221287-21-2-415
1959-10-01
2024-03-28
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References

  1. Abrams A. 1958; O-Acetyl groups in the cell wall of Streptococcus faecalis . J. biol. Chem 230:949
    [Google Scholar]
  2. Armstrong J. J., Baddiley J., Buchanan J. G., Carss B., Greenberg G. R. 1958; Isolation and structure of ribitol phosphate derivatives (teichoic acids) from bacterial cell walls. J. chem. Soc p. 4344
    [Google Scholar]
  3. Baddiley J., Buchanan J. G., Carss B. 1958; The presence of ribitol phosphate in bacterial cell walls. Biochim. biophys. Acta 27:220
    [Google Scholar]
  4. Buchanan J. G., Dekker C. A., Long A. G. 1950; The detection of glycosides and non-reducing carbohydrate derivatives in paper partition chromatography. J. chem. Soc p. 3162
    [Google Scholar]
  5. Cummins C. S., Harris H. 1956; The chemical composition of the cell wall in some Gram-positive bacteria and its possible value as a taxonomic character. J. gen. Microbiol 14:583
    [Google Scholar]
  6. Fiske C. E., SubbaRow Y. 1925; The colorimetric determination of phosphorus. J. biol. Chem 66:375
    [Google Scholar]
  7. Hestrin S. 1949; The reaction of acetylcholine and other carboxylic acid derivatives with hydroxylamine, and its analytical application. J. biol. Chem 180:249
    [Google Scholar]
  8. Ingram V. M., Salton M. R. J. 1957; The action of fluorodinitrobenzene on bacterial cell walls. Biochim. biophys. Acta 24:9
    [Google Scholar]
  9. Jermyn M. A., Isherwood F. A. 1949; Improved separation of sugars on the paper partition chromatogram. Biochem. J 44:402
    [Google Scholar]
  10. Johnson M. J. 1941; Isolation and properties of a pure yeast polypeptidase. J. biol. Chem 137:575
    [Google Scholar]
  11. Powell J. E. 1953; Isolation of dipicolinic acid (pyridine-2:6-dicarboxylic acid) from spores of Bacillus megatherium . Biochem. J 54:210
    [Google Scholar]
  12. Rondle C. J. M., Morgan W. T. J. 1955; The determination of glucosamine and galactosamine. Biochem. J 61:586
    [Google Scholar]
  13. Salton M. R. J. 1953; The composition of the cell walls of some Gram-positive and Gram-negative bacteria. Biochim. biophys. Acta 10:512
    [Google Scholar]
  14. Salton M. R. J. 1956; The action of lysozyme on cell walls of some lysozyme sensitive bacteria. Biochim. biophys. Acta 22:495
    [Google Scholar]
  15. Salton M. R. J. 1958; The lysis of micro-organisms by lysozyme and related enzymes. J. gen. Microbiol 18:481
    [Google Scholar]
  16. Salton M. R. J., Horne R. W. 1951; Methods of preparation and some properties of cell walls. Biochim. biophys. Acta 7:177
    [Google Scholar]
  17. Strange R. E., Dark F. A. 1956a; The composition of the spore coats of Bacillus megatherium, B. subtilis and B. cereus . Biochem. J 62:459
    [Google Scholar]
  18. Strange R. E., Dark F. A. 1956b; An unidentified amino-sugar present in cell walls and spores of various bacteria. Nature; Lond: 177186
    [Google Scholar]
  19. Work E. 1957; Reaction of ninhydrin in acid solution with straight-chain amino acids containing two amino groups and its application to the estimation of αƐ-diaminopimelic acid. Biochem. J 67:416
    [Google Scholar]
  20. Yoshida N., Izumi Y., Tani I., Tanaka S., Takaishi K., Hashimoto T., Fukui K. 1957; Studies on the chemical composition of bacterial cell walls and spore membranes. J. Bact 74:94
    [Google Scholar]
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