1887

Abstract

SUMMARY: The synthesis of cellulose by Acetobacter on media based on blackstrap molasses was studied. Methods are described for the determination of cellulose, and of growth as cell-N, in static culture. Different carbohydrate substrates were compared for cellulose production by strain EA-I; hydrolysed molasses was found to give the largest yields. In media based on this substrate growth and cellulose production both reached their maximum values with a sugar concentration of 7·9 % (w/v) after 40 days’ growth at 26–29°. The maximum conversion (18 %) of sugar to cellulose, however, was obtained with 1·5 (w/v) sugar. Cellulose synthesis ceased when growth stopped, even when the sugar in the medium was not exhausted, indicating that cellulose was synthesized only by the actively growing organism. The cellulose produced/mg. cell-N and % cellulose in the pellicles decreased with increased sugar concentration in the medium. Other Acetobacter strains were examined in this medium; the cellulose yields varied over a wide range, equivalent to conversions of 1·9–23·5 %.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-21-2-312
1959-10-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/21/2/mic-21-2-312.html?itemId=/content/journal/micro/10.1099/00221287-21-2-312&mimeType=html&fmt=ahah

References

  1. Barclay K. S. 1951 Studies on Bacterial Cellulose Ph.D.thesis University of Birmingham:
    [Google Scholar]
  2. Brown A. J. 1886; On an acetic ferment which forms cellulose. J. chem. Soc p. 432
    [Google Scholar]
  3. Dudman W. F. 1959; Cellulose production by Acetobacter acetigenum in defined medium. J. gen. Microbiol 21:327
    [Google Scholar]
  4. Fairbairn N. J. 1953; Modified anthrone reagent. Chem. & Ind p. 86
    [Google Scholar]
  5. Henneberg W. 1926 Handbuch der Gärungsbakteriologie Berlin: Parey; Vaughn. Cited by 1942
    [Google Scholar]
  6. Hestrin S., Feingold D. S., Schramm M. 1955 In Methods in Enzymology 1 p. 252 Colowick S. P., Kaplan N. O. Ed. by New York: Academic Press Inc;
    [Google Scholar]
  7. Hestrin S., Schramm M. 1954; Synthesis of cellulose by Acetobacter xylinum. 2. Preparation of freeze-dried cells capable of polymerizing glucose. Biochem. J 58:345
    [Google Scholar]
  8. Kaushal R., Walker T. K. 1951; Formation of cellulose by certain species of Acetobacter . Biochem. J 48:618
    [Google Scholar]
  9. Schramm M., Hestrin S. 1954; Factors affecting production of cellulose at the air/liquid interface of a culture of Acetobacter xylinum . J. gen. Microbiol 11:123
    [Google Scholar]
  10. Skopek J. 1955 Studies on the biological synthesis of cellulose by Acetobacter xylinum Ph.D. thesis University of Maryland: Doctoral dissertation series,Publication 12092, Ann Arbor, Mich: University Microfilms
    [Google Scholar]
  11. Somogyi M. 1952; Notes on sugar determination. J. biol. Chem 195:19
    [Google Scholar]
  12. Tare H. L. A., Hibbert H. 1931; Polysaccharide synthesis by the action of Acetobacter xylinus on carbohydrates and related compounds. Canad. J. Res 4:372
    [Google Scholar]
  13. Vaughn R. H. 1942; The acetic acid bacteria. Wallerstein Labs. Commun 5:5
    [Google Scholar]
  14. Woeber H. H. 1954 A biochemical study of cellulose synthesis by Acetobacter xylinum Ph.D. thesis University of Florida: Doctoral Dissertation Series Publication 9722, Ann Arbor, Mich: University Microfilms
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-21-2-312
Loading
/content/journal/micro/10.1099/00221287-21-2-312
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error