1887

Abstract

Summary: Upon repeated subculture in media of gradually increasing pH value a strain of developed the ability to grow under strongly akaline conditions. Organisms with the highest alkali resistance could grow at pH 10·3, and stocks of intermediate resistance were also obtained. When growing on alkaline agar, resistant bacteria underwent marked morphological changes. In liquid media, the bacteria retained the ability to synthesize lecithinase. Resistance was not lost upon several transfers on a neutral medium.

Resistant bacteria when growing in alkaline media caused a rapid decrease in pH value. A similar pH decrease was caused by sensitive bacteria in media of the highest degree of alkalinity in which they could grow. The decline of pH value accompanied, but did not precede, growth; acid production by the resistant bacteria does not appear to be a primary mechanism of resistance.

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/content/journal/micro/10.1099/00221287-21-1-96
1959-08-01
2024-12-13
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References

  1. Bryson V., Szybalski W. 1955; Microbial drug resistance. Advanc.Genetics 7:1
    [Google Scholar]
  2. Davis B. D. 1957; Physiological (phenotypic) mechanisms responsible for drug resistance. CIBA Foundation Symposium on Drug Resistance in Microorganisms, Mechanisms of Development p. 165 Eds. Wolstenholme G. W. E., O’Connor C. M. London: J. and A. Churchill Ltd.;
    [Google Scholar]
  3. Downie A. W., Cruickshank J. 1928; The resistance of Streptococcus faecalis to acid and alkaline media. Brit. J. exp. Path 9:171
    [Google Scholar]
  4. Gale E. F. 1948 The Chemical Activities of Bacteria New York: Academic Press Inc.;
    [Google Scholar]
  5. Heimpel A. M. 1954; A strain of Bacillus cereus Fr. and Fr. pathogenic for the larch sawfly, Pristiphora erichsonii (Htg.). Canad. Ent 86:73
    [Google Scholar]
  6. Heimpel A. M. 1955a; The pH in the gut and blood of the larch sawfly, Pristiphora erichsonii (Htg.), and other insects with reference to the pathogenicity of Bacillus cereus Fr. and Fr. Canad. J. Zool 33:99
    [Google Scholar]
  7. Heimpel A. M. 1955b; Investigations of the mode of action of strains of Bacillus cereus Fr. and Fr. pathogenic for the larch sawfly, Pristiphora erichsonii (Htg.). Canad. J. Zool 33:311
    [Google Scholar]
  8. Heimpel A. M., Angus T. A. 1958; Recent advances in the knowledge of some bacterial pathogens of insects. Proc. 10th Int. Congr. Entomol.Montreal 1956 4:711
    [Google Scholar]
  9. Kocholaty W., Hoogerheide J. C. 1938; Studies of the metabolism of the strict anaerobes (Genus: Clostridium). I. Dehydrogenation reactions by suspensions of Clostridium sporogenes. Biochem. J 32:437
    [Google Scholar]
  10. Kocholaty W., Weil L. 1938; Enzymic adaptation in Clostridium histolyticurn. . Biochem. J 32:1696
    [Google Scholar]
  11. Kushner D. J. 1957; An evaluation of the egg-yolk reaction as a test for lecithinase activity. J. Bact 73:297
    [Google Scholar]
  12. Kushner D. J., Heimpel A. M. 1957; Lecithinase production by strains of Bacillus cereus Fr. and Fr. pathogenic for the larch sawfly, Pristiphora erichsonii (Htg.). Canad. J. Microbiol 3:547
    [Google Scholar]
  13. Schnitzer R. J., Grunberg E. 1957 Drug Resistance of Microorganisms New York: Academic Press Inc.;
    [Google Scholar]
  14. Staudenmayer T. 1940; Die Wasserstoffionenkonzentration der Insekten. Anz. Schädlingsk 15:114
    [Google Scholar]
  15. Stephens J. M. 1952; Disease in codling moth larvae produced by several strains of Bacillus cereus. Canad. J. Zool 30:30
    [Google Scholar]
  16. Topley and wilson#x2019;s Principles of Bacteriology and Immunology 1955, 4th edition. p. 84 Eds. Wilson G. S., Miles A. A. London: Edward Arnold;
  17. Umbreit W. W. 1951; Significance of autotrophy for comparative physiology. Bacterial Physiology p. 566 Eds. Werkman C. H., Wilson P. W. New York: Academic Press Inc.;
    [Google Scholar]
  18. Waterhouse D. F. 1949; The hydrogen-ion concentration in the alimentary canal of larval and adult lepidoptera. Aust. J. Sci. Res.B 2:428
    [Google Scholar]
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