1887

Abstract

SUMMARY: A toxic protein has been isolated from Inaba and Ogawa strains of and from water vibrios by dissolving the bacteria in 2·5-urea and subsequent fractional precipitation with ammonium sulphate. This toxic protein accounted for the major portion of the toxicity of whole organism. Immunological and chemical data suggest that the toxic protein is the protein component of the Boivin antigen. The lipopolysaccharide endotoxins of the strains examined were relatively non-toxic as compared with the lipopolysaccharides from other Gramnegative bacteria, though in other biological and chemical properties they resembled the general class.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-21-1-191
1959-08-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/21/1/mic-21-1-191.html?itemId=/content/journal/micro/10.1099/00221287-21-1-191&mimeType=html&fmt=ahah

References

  1. Bernard P. N., Gallut J. 1945; Recherches sur la toxine du vibrion cholerique. Ann. Inst.Pasteur 71:65
    [Google Scholar]
  2. Boivin A., Mesrobeanu L. 1935; Recherches sur les antigenes somatiques et sur les endotoxines des bacteries. Rev. Immunol 1:553
    [Google Scholar]
  3. Burrows W. 1944; The endotoxin of the cholera vibrio: isolation and properties. Proc. Soc. exp. Biol., Med. N.Y 57:306
    [Google Scholar]
  4. Dische Z., Shettles L. B. 1948; A specific color reaction of methyl-pentoses and a spectrophotometric micromethod for their estimation. J. biol. Chem 175:595
    [Google Scholar]
  5. Dische Z., Shettles L. B., Osnos M. 1949; New specific color reactions of hexoses and spectrophotometric micromethods for their determination. Arch. Biochem 22:169
    [Google Scholar]
  6. Fiske C. H., Subbarow Y. 1925; The colorimetric determination of phosphorus. J. biol. Chem 66:375
    [Google Scholar]
  7. Freter R. 1956; Two different toxic fractions extracted from Vibrio cholerae. . J. infect. Dis 99:207
    [Google Scholar]
  8. Gallut J., Grabar P. 1945; Recherches immunochemique sur le vibrion cholérique. Ann. Inst.Pasteur 71:83
    [Google Scholar]
  9. Kabat E., Mayer M. M. 1948 Experimental Immunochemistry Springfield: C. C.Thomas;
    [Google Scholar]
  10. Markham R. 1942; A steam distillation apparatus suitable for micro-Kjeldahl apparatus. Biochem. J 36:790
    [Google Scholar]
  11. Reed L. J. 1938; A simple method of estimating fifty per cent endpoints. Amer. J. Hyg 27:493
    [Google Scholar]
  12. Rowley D. 1956; Induced changes in the level of non-specific immunity in laboratory animals. J. exp. Path 37:223
    [Google Scholar]
  13. Schwartzman G. 1937 The Phenomenon of Local Tissue Reactivity and its Immunological, Pathological and Clinical Significance New York: Paul Hoeber;
    [Google Scholar]
  14. Seifter S., Dayton S., Novic B., Muntyler E. 1950; Estimation of glycogen with the anthrone reagent. Arch. Biochem 25:191
    [Google Scholar]
  15. Smithies O. 1955; Zone electrophoresis in starch gels: group variations in the serum proteins of normal human adults. Biochem. J 61:629
    [Google Scholar]
  16. Stacy M., Kent P. W., Nassau E. 1951; Polysaccharide complexes from Mycobacterium tuberculosis (H) . Biochem. biophys.Acta 7:146
    [Google Scholar]
  17. Walker J. 1940; A method for the isolation of toxic and immunising fractions from bacteria of the salmonella group. Biochem. J 61:629
    [Google Scholar]
  18. Westphal O., Lüderitz O. 1954; Chemische Erforschung von Lipopoly- sacchariden gram negativer Bakterien. Angew. Chem 66:407
    [Google Scholar]
  19. Westphal O., Lüderitz O., Bister F. 1952; Über die Extraktion von Bakterien mit Phenol-Wasser. Z. Naturforsch 7b:148
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-21-1-191
Loading
/content/journal/micro/10.1099/00221287-21-1-191
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error