1887

Abstract

SUMMARY: -Oxoglutaric, pyruvic and amino acids accumulated extracellularly when was grown in a chemically defined medium containing glycerol as carbon source and ammonium sulphate as nitrogen source. The excretion of keto acids was diminished and that of amino acids was increased by raising the concentration of ammonium sulphate relative to that of glycerol. This change is consistent with the operation of a citric acid cycle which is performing synthetic functions. The labelling of the somatic amino acids formed in the presence of glycerol and either (C) bicarbonate or (2-C) acetate is also consistent with the occurrence of a citric acid cycle.

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1959-06-01
2021-10-25
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References

  1. Boissevain C. 1943; Survival of tubercle bacilli in solutions containing glycerol or its oxidation products. Proc. Soc. exp. Biol., N.Y 54:342
    [Google Scholar]
  2. Dagley S., Johnson A. R. 1956; Appearance of amino acids and peptides in culture filtrates of micro-organisms growing in mineral salt media. Biochim. Biophys. Acta 21:270
    [Google Scholar]
  3. Dent C. E. 1948; A study of the behaviour of some sixty amino-acids and other ninhydrin reacting substances on phenol-‘collidine’ filter-paper chromatograms, with notes as to the occurrence of some of them in biological fluids. Biochem. J 43:169
    [Google Scholar]
  4. Drea W. F., Andrejew A. 1953 The Metabolism, of the Tubercle Bacillus Illinois:: Charles C. Thomas.;
    [Google Scholar]
  5. Edson N. L., Hunter G.J.E. 1943; Respiration and nutritional requirements of certain members of the genus Mycobacterium . Biochem.J 37:563
    [Google Scholar]
  6. Edson N. L., Hunter G.J.E., Kulka R. G., Wright D. E. 1959; The intermediary metabolism of glycerol in Mycobacterium butyricum . Biochem.J (in the Press)
    [Google Scholar]
  7. El Hawary M.F.S., Thompson R.H.S. 1953; Separation and estimation of blood keto acids by paper chromatography. Biochem. J 53:563
    [Google Scholar]
  8. Friedemann T. E., Haugen G. E. 1943; Pyruvic acid. II. The determination of keto acids in blood and urine. J. biol. Chem 147:415
    [Google Scholar]
  9. Gale E. F. 1947; The assimilation of amino acids by bacteria. 1. The passage of certain amino-acids across the cell wall and their concentration in the internal environment of Streptococcus faecalis . J. gen. Microbiol 1:53
    [Google Scholar]
  10. Harrow B., Stone G.C.H., Borek E., Wagriech H., Mazur A. 1944 Laboratory Manual of Biochemistry U.S.A.:: W. Saunders and Co.;
    [Google Scholar]
  11. Hiller A., Plazin J., Van Slyke D. D. 1948; A study of conditions for Kjeldahl determination of nitrogen in proteins. Description of methods with mercury as catalyst, and titrimetric and gasometric measurements of the ammonia formed. J. biol. Chem 176:1401
    [Google Scholar]
  12. Hunter G.J.E. 1953; Acid formation in cultures of Mycobacterium butyricum . J. Bact 66:676
    [Google Scholar]
  13. Kogut M., Podoski E. P. 1953; Oxidative pathways in a fluorescent Pseudomonas . Biochem.J 55:800
    [Google Scholar]
  14. Lambert M., Neish A. C. 1950; Rapid method for estimation of glycerol in fermentation solutions. Canad. J. Res. B 28:83
    [Google Scholar]
  15. Lockwood L. B., Stodola F. H. 1946; Preliminary studies on the production of α-ketoglutaric acid by Pseudomonas fluorescens . J. biol. Chem 164:81
    [Google Scholar]
  16. Morrison N. E. 1956; Oxidation of malate by Mycobacterium smegmatis . Proc. Univ. Otago Med. Sch 34:23
    [Google Scholar]
  17. Morrison N. E., Wright D. E. 1958; Isocitratase activity in Mycobacterium butyricum . Proc. Univ. Otago Med. Sch 36:1–15
    [Google Scholar]
  18. Pauletta G., Defranceschi A. 1952; Studies on the amino acid metabolism of Mycobacterium tuberculosis. I. Primary investigation by paper chromatography. Biochim. Biophys. Acta 9:271
    [Google Scholar]
  19. Reid R. L., Lederer M. 1952; Separation and estimation of saturated C2-C7fatty acids by paper partition chromatography. Biochem. J 50:60
    [Google Scholar]
  20. Roberts R. B., Abelson P. H., Cowie D. B., Bolton E. T., Britten R. J. 1955; Studies of biosynthesis in Escherichia coli . Publ. Carneg. Instn 607:
    [Google Scholar]
  21. Shoji K., Yamagami A., Mori T. 1957; Studies on the amino acid metabolism of Mycobacterium tuberculosis. I. Utilisation of some nitrogenous substances and accumulation of free glutamic acid in bacterial cells. Med. J. Osaka Univ 7:787
    [Google Scholar]
  22. Yonemura T., Iizuka M. 1955a; Studies on the metabolism of amino acids in tubercle bacilli. I. The changes of free amino acids in liquid synthetic media following the growth of bacilli. Jap. J. Tuberc 3:6
    [Google Scholar]
  23. Yonemura T., Iizuka M. 1955b; Studies on the metabolism of amino acids in tubercle bacilli. II. On the mechanism of the appearance of free amino acids in the medium. Jap. J. Tuberc 3:22
    [Google Scholar]
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