SUMMARY: The growth characteristics of eight strains of were studied in defined liquid media. Two of these strains (1017, 1018), which are both very virulent on intracerebral injection into mice, were found to have growth idiosyncrasies. Both these strains differed from typical virulent pertussis strains and from a degraded strain in that they needed more magnesium and a different gas phase (carbon dioxide and oxygen) for optimal growth in defined liquid medium. Both strains were inhibited by crystalline lysozyme (100 mg./ml.) in defined medium, whereas typical virulent strains and a degraded strain were insensitive to it.


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