1887

Abstract

SUMMARY: A total of 978 cultures of heterotrophic organisms were isolated from twelve actively nitrifying soils; each isolate was tested for ability to form nitrite or nitrate in glucose peptone broth. None of the isolates yielded substantial amounts of nitrite; concentrations found did not exceed 2 g. nitrite-N/ml. Almost 7 % of the isolates formed nitrite-N in excess of 0·2 g./ml., while slightly over 2 yielded more than 0·5 g./ml. Fungus isolates were the most numerous and most active nitrite producers; fifteen of the fungi formed nitrate in addition to nitrite. Concentrations of 5–45 g. nitrate-N/ml. were recorded for the active fungi. Further isolations of fungi from ten soils of diverse properties resulted in 353 cultures but only three of these formed nitrate. One of these cultures yielded 90 g. nitrate-N/ml. after 14 days growth in the test medium.

Most of the fungi which produced nitrate (16 of the 18 active cultures) were identified as A sp., and a sp. also formed low concentrations of nitrate. Each isolate of obtained from soil proved capable of nitrate formation. Strains of were encountered which did not yield nitrate, but these had been obtained from culture collections, and had been carried on artificial media for many years. Some stock cultures of several cultures of and one of freshly isolated from wheat seed, also were active nitrate producers. Ammonium sulphate and urea media supported growth of soil fungus isolates but not nitrate formation. Other organic nitrogen substrates in order of increasing effectiveness in nitrate production were: yeast extract, peptone, Protone and casein.

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1959-06-01
2024-04-24
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References

  1. Barritt N. W. 1933; The nitrification process in soils and biological filters. Arm. appl. Biol 20:165
    [Google Scholar]
  2. Fisher T., Fisher E., Appleman M. D. 1956; Nitrite production by heterotrophic bacteria. J. gen. Microbiol 14:238
    [Google Scholar]
  3. Hutton W. E., ZoBell C. E. 1953; Production of nitrite from ammonia by methane oxidizing bacteria. J. Bact 65:216
    [Google Scholar]
  4. Isenberg H. D., Schatz A., Angrist A. A., Schatz V., Trelawny G. S. 1954; Microbial metabolism of carbamates. II. Nitrification of urethan by Streptomyces nitrificans . J. Bact 68:5
    [Google Scholar]
  5. Jensen H. L. 1951; Nitrification of oxime compounds by heterotrophic bacteria. J. gen. Microbiol 5:360
    [Google Scholar]
  6. Martin J. P. 1950; Use of acid, rose bengal and streptomycin in the plate method for estimating soil fungi. Soil Sci 69:215
    [Google Scholar]
  7. Meiklejohn J. 1953; The nitrifying bacteria: A review. J. Soil Sci 4:59
    [Google Scholar]
  8. Quastel J. H., Scholefield P. G. 1949; Influence of organic nitrogen compounds on nitrification in soil. Nature; Lond: 1641068
    [Google Scholar]
  9. Quastel J. H., Scholefield P. G., Stevenson J. W. 1950; Oxidation of pyruvic oxime by soil organisms. Nature; Lond: 166940
    [Google Scholar]
  10. Quastel J. H., Scholefield P. G., Stevenson J. W. 1952; Oxidation of pyruvic oxime by soil organisms. Biochem. J 51:278
    [Google Scholar]
  11. Schatz A., Isenberg H. D., Angrist A. A., Schatz V. 1954; Microbial metabolism of carbamates. I. Isolation of Streptomyces nitrificans, n.sp., and other organisms which grow on urethan. J. Bact 68:1
    [Google Scholar]
  12. Schmidt E. L. 1954; Nitrate formation by a soil fungus. Science 119:187
    [Google Scholar]
  13. Schmidt E. L. 1956; Soil nitrification and nitrates in waters. Publ. Hlth Rep 71:497
    [Google Scholar]
  14. Schmidt E. L., Ruschmeyer O. R. 1958; Cellulose decomposition in soil burial beds. I. Soil properties in relation to cellulose degradation. Appl. Microbiol 6:108
    [Google Scholar]
  15. Snell F. D., Snell C. T. 1949 Colorimetric Methods of Analysis II, third edition. New York: D. van Nostrand;
    [Google Scholar]
  16. Stephenson M. 1939 Bacterial Metabolism, second edition. London: Longmans, Green and Co;
    [Google Scholar]
  17. Thom C., Raper K. B. 1945 A Manual of the Aspergilli Baltimore, Md., U.S.A: Williams and Wilkins;
    [Google Scholar]
  18. Waksman S. A. 1946; Sergi Nikolaevitch Winogradsky. Soil Sci 62:197
    [Google Scholar]
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