1887

Abstract

SUMMARY: Sodium-rich yeast was prepared by aerating a yeast suspension in -NaCl for a period of 60 min. This yeast contained then on the average 0·070–0·075 m- equiv. Na/g. dry wt. On incubating it with glucose, xylose or lactose in water, 0·1 -KCI or 0·1 -NaCl it lost some of its sodium, in the last case against an apparent concentration gradient. It appears that this movement of Na ion is of a diffusion character as evidenced by the effect of temperature, by incubation without metabolizable substrates and by the use of metabolic inhibitors; however, the possibility of an active contribution to this efflux cannot be excluded. The movement of Na+ out of the cell was shown to be connected with considerable changes of cell volume effected above all by changes of osmotic pressure of the medium, sodium being lost as a 0·06-0·10 solution. A discussion of these phenomena is presented.

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/content/journal/micro/10.1099/00221287-20-2-197
1959-04-01
2022-05-29
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References

  1. Burger M., Hejmová L., Kleinzeller A. 1959; Transport of some mono- and disaccharides into yeast cells. Biochem. J 71:233
    [Google Scholar]
  2. Conway E. J., Brady T. G. 1950; Biological production of acid and alkali. Biochem. J 47:360
    [Google Scholar]
  3. Conway E. J., Downey M. 1950; An outer metabolic region of the yeast cell. Biochem. J 47:347
    [Google Scholar]
  4. Conway E. J., Moore P. T. 1954; A sodium yeast and some of its properties. Biochem. J 57:523
    [Google Scholar]
  5. Conway E. J., Ryan H., Carton E. 1954; Active transport of sodium ions from the yeast cell. Biochem. J 58:158
    [Google Scholar]
  6. Cort J. H., Kleinzeller A. 1958; The effect of temperature on the transport of sodium and potassium by kidney cortex slices. J. Physiol 142:208
    [Google Scholar]
  7. Glynn I. M. 1957; The ionic permeability of the red cell membrane. Progr. Biophys 8:241
    [Google Scholar]
  8. Kotyk A. 1958; The influence of K+ on phosphorylation processes in Saccharomyces cerevisiae . Biokhimija 23:737
    [Google Scholar]
  9. Rothstein A., Enns L. H. 1946; The relation of K to carbohydrate metabolism in baker’s yeast. J. cell. comp. Physiol 28:231
    [Google Scholar]
  10. Werkheiser W. C., Bartley W. 1957; The study of steady state concentrations of internal solutes of mitochondria by rapid centrifugal transfer to a fixation medium. Biochem. J 66:79
    [Google Scholar]
  11. White J. 1954 Yeast Technology London: Chapman and Hall;
    [Google Scholar]
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