@article{mbs:/content/journal/micro/10.1099/00221287-20-1-43, author = "Eberhart, B. M. and Tatum, E. L.", title = "A Gene Modifying the Thiamine-requirement of Strains of Neurospora crassa", journal= "Microbiology", year = "1959", volume = "20", number = "1", pages = "43-53", doi = "https://doi.org/10.1099/00221287-20-1-43", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-20-1-43", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Summary: A new gene, thi-1o, is described in Neurospora crassa. This gene is shown to be a modifier of the nutritional response of strains containing the thi-1 gene, and is not nutritionally detectable in the absence of the thi-1 mutant locus. The gene thi-1o is additionally characterized by a decreased ability to synthesize thiamine from thiazole and pyrimidine; thi-1o appears to modify only thi-1 strains because of their unusually high thiamine requirements. The nutritional responses of strains containing either thi-2 or thi-3 loci are not affected by the presence of thi-1o. Both thi-1o and thi-4 are anomalous in the condensation of pyrimidine and thiazole. The probability that thi -1o may be an isoallele of thi-4 is based upon a similarity of metabolic pattern and upon location on chromosome III. The failure to obtain recombination in crosses between strains containing thi-1o and thi-4 constitutes additional support for their allelism.", }