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Abstract
SUMMARY: The hypothesis that halophilic bacteria achieve a high degree of salt tolerance by exclusion of much of the external solutes of the growth medium has been tested. Organisms were grown in media containing from 1 to 4 m salts. For both halophilic and non-halophilic bacteria the freezing points of the cells were close to those of the media in which they were grown. It is concluded that halophilic bacteria do not maintain the intracellular aqueous phase at a water activity greater than in the surrounding growth medium.
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© Society for Gerenal Microbiology, 1959