The Microbioloǵical Estimation of Nicotinic Acid and Comparison with a Chemical Method Free

Abstract

Summary: A modified basal medium was employed for the assay of nicotinic acid contained in various biological materials. Of the various possible comparisons of test material with standard nicotinic acid in the assay, the ‘internal standard’ method is preferable; by assaying the standard nicotinic acid in the presence of the test material, any discrepancies due to substances stimulating or inhibiting the nicotinic acid effect are minimized.

Of the different extraction procedures tested, treatment of samples with -NaOH and -HSO gave the most satisfactory results; both -NaOH and -HSO appeared to convert a ‘precursor’ in potato and bran, into nicotinic acid. 2-HCl extracts yielded lower values, but the conversion of the precursor was less effective with potato than with bran, suggesting different precursors in the two vegetable substances.

The results of microbiological and chemical assay were in good agreement. The coefficient of variation of the microbiological results was of the order of 9%.

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/content/journal/micro/10.1099/00221287-2-3-306
1948-09-01
2024-03-29
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References

  1. Andrews J. S., Boyd H. M., Gortner W. A. 1942; Nicotinic acid content of cereals and cereal products. Industr. Engng. Chem (Anal, ed.) 14:663
    [Google Scholar]
  2. Barton-Wright E. C. 1945; The theory and practice of the microbiological assay of the vitamin-B complex; together with the assay of selected amino-acids and potassium. Analyst 70:283
    [Google Scholar]
  3. Kodicek E. 1940; Estimation of nicotinic acid in animal tissues, blood and certain foodstuffs. Biochem. J 34:712–724
    [Google Scholar]
  4. Kodicek E., Pepper C. R. 1948; A critical study of factors influencing the microbiological assay of nicotinic acid. J. gen. Microbiol 2:292
    [Google Scholar]
  5. Krehl W. A., Huerga J., Elvehjem C. A., Hart E. B. 1946; The distribution of niacinamide and niacin in natural materials. J. biol. Chem 166:53
    [Google Scholar]
  6. Krehl W. A., Strong F. M. 1944; Studies on the distribution, properties, and isolation of a naturally occurring precursor of nicotinic acid. J. biol. Chem 156:1
    [Google Scholar]
  7. Oser B. L., Melnick D., Siegel L. 1941; Control of nicotinic acid in flour and bread. Food Industr 13:66
    [Google Scholar]
  8. Snell E. E., Strong F. M. 1939; A microbiological assay for riboflavin. Industr. Engng. Chem (Anal, ed.) 11:346
    [Google Scholar]
  9. Snell E. E., Wright L. D. 1941; A microbiological method for the determination of nicotinic acid. J. biol. Chem 139:675
    [Google Scholar]
  10. Waisman H. A., Elvehjem C. A. 1941; Chemical estimation of nicotinic acid and vitamin B6 . Industr. Engng. Chem (Anal, ed.) 13:221
    [Google Scholar]
  11. Wang Y. L., Kodicek E. 1943; Vitamin methods. A new modification of the p-aminoacetophenone method for estimating nicotinic acid in urine. Biochem. J 37:530
    [Google Scholar]
  12. Wood E. C. 1945; Calculations of the results of microbiological assays. Nature; Lond: 155632
    [Google Scholar]
  13. Wood E. C. 1946; The theory of certain analytical procedures, with particular reference to micro-biological assays. Analyst 71:1
    [Google Scholar]
  14. Wood E. C., Finney D. J. 1946; The design and statistical analysis of microbiological assays. Quart. J. Pharm 19:112
    [Google Scholar]
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