1887

Abstract

Summary: The cultural characters of a strain of and used in the microbiological assay of riboflavin and nicotinic acid proved to be typical, excepting that neither strain fermented xylose and the strain of fermented rhamnose but not raffinose. Neither produced catalase, so that a strongly positive catalase test in these cultures indicates probable contamination by common air-borne micro-organisms.

Modifications of the medium to achieve maximum acid production were investigated by altering, omitting or adding various constituents. A modified medium was adopted allowing a slightly higher acid production. In this medium the maximum acid production was obtained in the presence of natural substances like peptone. The maximal acid production obtainable with nicotinic acid alone was slightly lower, suggesting an additional specific factor in peptone. No confirmatory evidence, however, could be obtained of its existence. Linoleic acid in a concentration of 640 g./10 ml. medium depressed the acid production, and its action was antagonized by cholesterol. Other fatty acids and lipids had no effect.

A good reproducibility and a small coefficient of variation was found between tubes at various levels of nicotinic acid within any one assay, but between separate assays the variation was high (coefficient of variation = 12–19%).

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-2-3-292
1948-09-01
2021-07-31
Loading full text...

Full text loading...

/deliver/fulltext/micro/2/3/mic-2-3-292.html?itemId=/content/journal/micro/10.1099/00221287-2-3-292&mimeType=html&fmt=ahah

References

  1. Barton-Wright E.C. 1944; The microbiological assay of nicotinic acid in cereals and other products. Biochem. J 38:314
    [Google Scholar]
  2. Barton-Wright E.C. 1945; The theory and practice of the microbiological assay of the vitamin-B complex; together with the assay of selected amino-acids and potassium. Analyst 70:283
    [Google Scholar]
  3. Bergey’S 1939 Manual of Determinative Bacteriology. Baltimore:, 5th ed..
    [Google Scholar]
  4. Camien M.N., Dunn M.S., Salle A.J. 1947; The effect of carbohydrates on acid production by twenty-four lactic acid bacteria. J. biol. Chem 168:33
    [Google Scholar]
  5. Chattaway F.W., Happold F.C., Sandford M. 1943; The microbiological assay of riboflavin. The influence of inorganic constituents and unknown growth factors. Biochem. J 37:298
    [Google Scholar]
  6. Day A.A., Gibbs W.M. 1928; Surface tension in relation to bacterial growth with special reference to Lactobacillus acidophilus and Lactobacillus bulgaricus. J. infect. Dis 43:97
    [Google Scholar]
  7. Guirard B.M., Snell E.E., Williams R.J. 1946; The nutritional role of acetate for lactic acid bacteria. I. The response to substances related to acetate. Arch. Biochem 9:361
    [Google Scholar]
  8. Isbell H. 1945; The use of charcoal-treated peptone in microbiological assays. Science 102:671
    [Google Scholar]
  9. Knight B.C.J.G. 1945; Growth factors in microbiology. Some wider aspects of nutritional studies with micro-organisms. Vitamins and Hormones 3105 N.Y.: Academic Press, Inc;
    [Google Scholar]
  10. Kodicek E. 1948; The microbiological assay of riboflavin. (In the Press)
    [Google Scholar]
  11. Kodicek E., Pepper C.R. 1948; The microbiological estimation of nicotinic acid and comparison with a chemical method. J. gen. microbiol 2:306
    [Google Scholar]
  12. Kodicek E., Worden A.N. 1945; The effect of unsaturated fatty acids on Lactobacillus helveticus and other Gram-positive micro-organisms. Biochem. J 39:78
    [Google Scholar]
  13. Kodicek E., Worden A.N. 1946; Effect of unsaturated fatty acids on the acid production of Lactobacillus helveticus. Nature; Lond: 157587
    [Google Scholar]
  14. Krehl W.A., Strong F.M., Elvehjem C.A. 1943; Determination of nicotinic acid. Modification in the microbiological method. Industr. Engng Chem. Anal, ed. 15:471
    [Google Scholar]
  15. Leonian L.H., Lilly V.G. 1945; The comparative value of different test organisms in the microbiological assay of B vitamins. Bull. W. Va agric. Exp. Sta.31935
    [Google Scholar]
  16. McIntosh J., James W.W., Barlow P.L. 1922; An investigation into the etiology of dental caries. I. The nature of the destructive agent and the production of artificial caries. Brit. J. exp. Path 3:138
    [Google Scholar]
  17. Pennington D. 1946; Assay of p-aminobenzoic acid. Science 103:397
    [Google Scholar]
  18. Pepper C.R. 1947; Microbiological studies on certain B-vitami. M.Sc. Thesis University of Cambridge:
    [Google Scholar]
  19. Pollack M.A., Lindner M. 1943; A growth stimulant for Lactobacillus casei. J. biol. Chem 147:183
    [Google Scholar]
  20. Sarett H.P., Pederson R.L., Cheldelin V.H. 1945; Microbiological assay methods for nicotinic acid. Arch. Biochem 7:77
    [Google Scholar]
  21. Shankman S. 1943; Amino-acid nutrition of Lactobacillus arabinosus. J. biol. Chem 150:305
    [Google Scholar]
  22. Shankman S., Camien M.N., Block H., Merrifield R.B., Dunn M.S. 1947; Vitamin requirements of twenty-three lactic acid bacteria. J. biol. Chem 168:23
    [Google Scholar]
  23. Snell E.E. 1945; The nutritional requirements of the lactic acid bacteria and their application to biochemical research. J. Bact 50:373
    [Google Scholar]
  24. Snell E.E., Strong F.M. 1938; The influence of riboflavin and certain synthetic flavins on the growth of lactic acid bacteria. J. biol. Chem 123: cxii
    [Google Scholar]
  25. Snell E.E., Strong F.M. 1939; A microbiological assay for riboflavin. Industr. Engng Chem.(Anal, ed.) 11:346
    [Google Scholar]
  26. Snell E.E., Wright L.D. 1941; A microbiological method for the determination of nicotinic acid. J. biol. Chem 139:675
    [Google Scholar]
  27. White B., Avery O.T. 1910; Observations of certain lactic acid bacteria of the so-called Bulgaricus type. Zbl. Bakt (2. Abt.) 25:161
    [Google Scholar]
  28. Wilson G.S., Miles A.A. 1946 Topley and Wilson’s Principles of Bacteriology and Immunity p. 752 London: Edward Arnold and Co;
    [Google Scholar]
  29. Woolley J.G., Sebrell W.H. 1945; Two microbiological methods for the determination of l( – )-tryptophane in proteins and other complex substances. J. biol. Chem 157:141
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-2-3-292
Loading
/content/journal/micro/10.1099/00221287-2-3-292
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error