Abstract
Summary: Glutamine is not required for staphylococcal growth, and was found to be synthesized by staphylococci from NH3 and glutamic acid in the presence of glucose. The amount of glutamine found was, however, much less than that of the NH3 reacting. Similar relationships were found in Proteus morganii, Pr. vulgaris, Saccharomyces cerevisiae and S. ellipsoideus. Proteus morganii was, however, outstanding in possessing a powerful glutaminase which could easily be separated from the cells; in this it differed markedly from Pr. vulgaris. Glutaminase activity was also demonstrated in yeasts, where its occurrence has been previously a matter of dispute.
Received:
11/12/1947
Published Online:
Sat May 01 00:00:00 UTC 1948
© Society for General Microbiology, 1948
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References
Archibald R.M.
1945 ; Chemical characteristics and physiological rftles of glutamine. Chem. Rev. 37:161
[Google Scholar]
Fildes P. ,
Gladstone G.P.
1939 ; Glutamine and the growth of bacteria. Brit. J. exp. Path. 20:334
[Google Scholar]
Fildes P. ,
Richardson G.M.
1937 ; Nutrition of Staphylococcus aureus: sulphur requirements. Brit. J. exp. Path. 18:292
[Google Scholar]
Friedemann T.E. ,
Graeser J.B.
1933 ; The determination of lactic acid. J. biol. Chem. 100:291
[Google Scholar]
Gale E.F.
1947 ; Passage of amino-acids across cell walls. J. gen. Microbiol. 1:53
[Google Scholar]
Geddes W.F. ,
Hunter A.
1928 ; Asparaginase. J. biol. Chem. 77:197
[Google Scholar]
Gladstone G.P.
1939 ; Interrelationships between amino-acids in the nutrition of B. anthracis . Brit. J. exp. Path. 20:189
[Google Scholar]
Grassmann W. ,
Mayr O.
1933 ; Zur Kenntnis der Hefeasparaginase. Hoppe-Seyl. Z. 214:185
[Google Scholar]
Krebs H.A.
1948 Biochem. J. 43:51
[Google Scholar]
Krebs H.A. ,
Eggleston L.V.
1940 ; Pyruvate oxidation in muscle. Biochem. J. 34:442
[Google Scholar]
Lipmann F. ,
Tuttle L.C.
1944 ; Acetyl phosphate: chemistry, determination and synthesis. J. biol. Chem. 153:571
[Google Scholar]
McIlwain H.
1946a ; Ammonium formation from glutamine by haemolytic streptococci. Biochem. J. 40:67
[Google Scholar]
McIlwain H.
1946b ; Products of the decomposition of glutamine during streptococcal glycolysis. Biochem. J. 40:460
[Google Scholar]
McIlwain H. ,
Fildes P. ,
Gladstone G.P. ,
Knight B.C.J.G.
1939 ; Glutamine and the growth of Streptococcus haemolyticus . Biochem. J. 33:223
[Google Scholar]
McIlwain H. ,
Hughes D.E.
1945 ; Relationships between metabolic and growth inhibitions by pantothenate analogues. Biochem. J. 39:133
[Google Scholar]
McIlwain H. ,
Roper J.A. ,
Hughes D.E.
1948 ; Growth and glutamine metabolism. Biochem. J. 42:492
[Google Scholar]
Nielsen N.
1941 ; Erganzende Untersuchungen iiber die Wuchsstoffwirkung der Aminosuaren auf Hefe. Biochem. Z 307:187
[Google Scholar]
Roper J.A. ,
McIlwain H.
1948 ; Glutamic acid analogues and glutamine assay. Biochem. J. 42:485
[Google Scholar]
Schwab G.
1936 Planta 25:579 Quoted by
Archibald R.M. 1945
[Google Scholar]
Smythe C.V.
1939 ; The effect of certain tissue extracts, of ammonium salts and of certain amides on the rate of fermentation by baker’s yeast. Enzymologia 6:9
[Google Scholar]
Taylor E.S. 1947 ; Free amino-acids in bacteria. J. gen. Microbiol. 1:86
[Google Scholar]
Vickery H.B. ,
Pucher G.W. ,
Clark H.E. ,
Chibnall A.C. ,
Westall R.G.
1935 ; Determination of glutamine in the presence of asparagine. Biochem. J 29:271
[Google Scholar]
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