SUMMARY: The influence of certain carbohydrates, nitrogen compounds and accessory substances on the growth and sporulation of and has been studied. It has been confirmed that needs an external supply of biotin, which has also a slight effect on the growth of For , a very low level of soluble sugar in the medium was essential for the production of perithecia. With adequate biotin, sporulated in the presence of considerable concentrations of sugar, but at low biotin levels no sporulation occurred until soluble sugar approached exhaustion.

Jute extract stimulated growth and accelerated sporulation of ; this was not due to the presence in the extract of any of nine well-known B-group vitamins. Jute extract had no more influence on than would be due to its biotin content.


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