1887

Abstract

SUMMARY: An inhibitor of plant viruses can be isolated from the sap of by differential precipitation with ethanol followed by adsorption on celite and elution with 10% NaCl. Purified preparations contain 14–15% nitrogen and 8–12% carbohydrate and the inhibitor is probably a glycoprotein. Denaturation leads to loss of inhibiting power. The protein, unless denatured, is unaffected by pepsin and trypsin.

The glycoprotein is isoelectric at about pH 7. It can combine with tobacco mosaic virus, and when salt-free solutions of the two are mixed in certain proportions at pH values between their isoelectric points it precipitates the virus in the form of paracrystalline threads. The glycoprotein also precipitates tomato bushy stunt virus.

When added to several plant viruses, the glycoprotein causes an immediate reduction in infectivity, but has no effect on a bacteriophage. Non-infective mixtures regain infectivity when diluted. No evidence was found for a combining ratio of virus to inhibitor necessary to cause loss of infectivity. The mechanism of virus neutralization is discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-2-2-143
1948-05-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/2/2/mic-2-2-143.html?itemId=/content/journal/micro/10.1099/00221287-2-2-143&mimeType=html&fmt=ahah

References

  1. Bawden F.C. 1943 Plant Viruses and Virus Diseases. Waltham, Mass.: Chronica Botanica Co;
    [Google Scholar]
  2. Bawden F.C., Pirie N.W. 1937; The isolation and some properties of liquid crystalline substances from solanaceous plants infected with three strains of tobacco mosaic virus. Proc. Roy. Soc. B 123:274
    [Google Scholar]
  3. Bawden F.C., Pirie N.W. 1943; Methods for the purification of tomato bushy stunt and tobacco mosaic viruses. Biochem. J 37:60
    [Google Scholar]
  4. Caldwell J. 1933; The physiology of virus diseases in plants. Ann. appl. Biol 20:100
    [Google Scholar]
  5. Caldwell J. 1938; Factors affecting the formation of local lesions by tobacco mosaic virus. Proc. Roy. Soc. B 119:493
    [Google Scholar]
  6. Chester K.S. 1934; Specific quantitative neutralization of the viruses of tobacco mosaic, tobacco ring spot and cucumber mosaic by immune sera. Phytopathology 24:1180
    [Google Scholar]
  7. Fulton R.W. 1943; The sensitivity of plant viruses to certain inactivators. Phytopathology, 33:674
    [Google Scholar]
  8. Grant T.J. 1934; The host range and behaviour of the ordinary tobacco mosaic virus. Phytopathology, 24:811
    [Google Scholar]
  9. Kleczkowska J. 1945; The production of plaques by Rhizobiumbacteriophage in poured plates and its value as a counting method. J. Bact 50:71
    [Google Scholar]
  10. Kleczkowski A. 1944; Combination of potato virus X and tobacco mosaic virus with pepsin and trypsin. Biochem. J. 38:160
    [Google Scholar]
  11. Kleczkowski A. 1946; Combination between different proteins and between proteins and yeast nucleic acid. Biochem. J 40:677
    [Google Scholar]
  12. Kunitz M. 1940; Crystalline ribonuclease. J. gen. Physiol 24:15
    [Google Scholar]
  13. Kuntz J.E., Walker J.C. 1947; Virus inhibition by extracts of spinach. Phytopathology, 37:561
    [Google Scholar]
  14. Loring H.S. 1942; The reversible inactivation of tobacco mosaic virus by crystal-fine ribonuclease. J. gen. Physiol 25:497
    [Google Scholar]
  15. Miyake S. 1927; Die isoelektrischen Punkte der Protamine. Hoppe-Seyl. Z 172:225
    [Google Scholar]
  16. Neurath H., Greenstein J.P., Putnam F.W., Erickson J.O. 1944; The chemistry of protein denaturation. Chem. Rev 34:157
    [Google Scholar]
  17. Stanley W.M. 1934; Chemical studies on the virus of tobacco mosaic. I. Some effects of trypsin. Phytopathology 24:1055
    [Google Scholar]
  18. Takahashi W.N. 1942; A virus inactivator from yeast. Science 95:586
    [Google Scholar]
  19. Takahashi W.N. 1946; Properties of a virus inactivator from yeast. Science 104:377
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-2-2-143
Loading
/content/journal/micro/10.1099/00221287-2-2-143
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error