1887

Abstract

Summary: The optimum water activity ( ) for survival of dried bacteria has been determined by storing the organisms in water-vapour equilibrium with solutions of known value. The position of the optimum differed according to the composition of the suspending fluid, and the presence or absence of air in the storage atmosphere. For and , dried in papain digest and stored , survival at 0·07, 0·11 and 0·16 was somewhat better than at 0·00 and 0·22 . Survival at 0·33–0·53 was much less. For dried in the same medium the optimum, was less clearly defined, but was well marked and close to 0·2 when stored in air. , when stored , survived best at 0·22 after drying in a salts buffer or in dialysed horse serum, but in whole serum or the dialysable serum fraction survival was better at 0·00 . dried from water survived best at 0·00 , in vacuo, but at 0·43 in air. In the presence of sucrose the effect of , was relatively small. In the presence of glucose or arabinose survival at 0·00 , was better than at 0·22 , which in turn was much better than at 0·48 . In the presence of all three sugars the differences between results and in air were small.

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/content/journal/micro/10.1099/00221287-19-3-624
1958-12-01
2021-10-21
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References

  1. Bullock K., Lightbown J.W. 1947; The inactivation of enzymes and micro-organisms in oils and powders. II. The effect of drying on the viability of bacteria, and their thermolability in powders. Quart. J. Pharm 20:312
    [Google Scholar]
  2. Fry R.M. 1954; The preservation of bacteria. p. 215 in Biological Applications of Freezing and Drying New York: Academic Press;
    [Google Scholar]
  3. Fry R.M., Greaves R.I.N. 1951; The survival of bacteria during and after drying. J. Hyg., Carrib 49:220
    [Google Scholar]
  4. Hutton R.S., Hilmoe R.J., Roberts J.L. 1951; Some physical factors that influence the survival of Brucella abortus during freeze-drying. J. Bact 61:309
    [Google Scholar]
  5. Naylor H.B., Smith P.A. 1946; Factors affecting the viability of Serratia marcescens during dehydration and storage. J. Bact 52:565
    [Google Scholar]
  6. Proom H., Hemmons L.M. 1949; The drying and preservation of bacterial cultures. J. gen. Microbiol 3:7
    [Google Scholar]
  7. Rhodes M. 1950; Viability of dried bacterial cultures. J. gen. Microbiol 4:450
    [Google Scholar]
  8. Robinson R.A., Stokes R.H. 1955 Eledrolyte Solutions London: Butter-worth;
    [Google Scholar]
  9. Scott W.J. 1958; Water relations of Staphylococcus aureus at 30°C. Aust. J. biol. Set 6:549
    [Google Scholar]
  10. Scott W.J. 1957; Water relations of food spoilage micro-organisms. Advanc. Food Res 7:83
    [Google Scholar]
  11. Scott W.J. 1958; A mechanism causing death during storage of dried micro-organisms. Symposium on Freezing and Drying London: Institute of Biology;
    [Google Scholar]
  12. Stamp, Lord 1947; The preservation of bacteria by drying. J. gen. Microbiol 1251
    [Google Scholar]
  13. Watts P.S. 1945; The effect of humidity on the survival of dried cultures of Streptococcus agalactiae. J. Path. Bact 57:191
    [Google Scholar]
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