The influence of the temperature of incubation on the course of formic acid and gas production by cultures of a coliform organism growing in buffered and unbuffered peptone media was studied. The evidence suggests that at 30° and at 37° the onset of gas production is determined by the concentration of undissociated formic acid in the culture, but that a higher concentration of acid is required to elicit response at 37° than at 30°. Undissociated formic acid is toxic to the organism and it appears that gas is not produced in unbuffered glucose medium at 37° because the environmental conditions do not allow elaboration of hydrogenlyase before inhibitory conditions of pH value and formate concentration are attained.


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