A method was found for infecting all the cells of the de-embryonated hen's egg with influenza B virus (strain Lee). Infected cells took up more glucose from the culture medium than did uninfected cells. Neither infected nor uninfected cells took up any of six amino acids, given singly. A mixture of thirteen amino acids was taken up well, and to the same extent, by infected and uninfected cells. When cysteine, histidine or methionine was omitted from this mixture, the remaining amino acids were taken up better by uninfected cells than by infected cells; when any other amino acid (arginine, glutamine, isoleucine, leucine, lysine, phenylalanine, threonine, tryptophan, tyrosine, valine) was omitted there was no significant difference in uptake by infected and uninfected cells. In this system, ethionine delayed multiplication of influenza virus, but β-phenylserine did not. These results are consistent with an increased requirement of the infected cell in this system for cysteine, histidine and methionine.


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