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Abstract
SUMMARY: Biotin and methionine are essential growth factors for the aerobic thermophilic actinomycete Micromonospora vulgaris. Biotin can be partially replaced by Tween 80 (sorbitan mono-oleate). No aerial growth is obtained in the absence of methionine. A chemically defined medium containing mineral salts, phosphate buffer (pH 6·8), soluble starch, a mixture of 18 synthetic amino acids (including methionine) and biotin will support growth of the organism.
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© Society for Gerenal Microbiology, 1958