The Induced Synthesis of Hydrogenase by Free

Abstract

SUMMARY: lack hydrogenase activity when grown hetero- trophically, but these organisms develop hydrogenase activity when incubated, in phosphate buffer, under an atmosphere of 95 % (v/v) hydrogen + 5 % (v/v) air. The development of hydrogenase activity is inhibited by 2:4-dinitrophenol and by chloramphenicol. The development is also inhibited by the addition of sodium lactate or sodium acetate unless a nitrogen source (e.g. ammonium sulphate) is present.

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/content/journal/micro/10.1099/00221287-19-2-223
1958-10-01
2024-03-29
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References

  1. Atkinson D. E., McFadden B. A. 1954; The biochemistry of Hydrogenomonas. I. The hydrogenase of Hydrogenomonas facilis in cell-free preparations. J. biol. Chem 210:885
    [Google Scholar]
  2. Hughes D. E. 1951; A press for disrupting bacteria and other micro-organisms. Brit. J. exp. Path 32:97
    [Google Scholar]
  3. Kistner A. 1954; Conditions determining the oxidation of carbon monoxide and of hydrogen by Hydrogenomonas carboxydovorans. Proc. Acad. Set. Amst 57c:186
    [Google Scholar]
  4. Kluyver A. J., Manten A. 1942; Some observations on the metabolism of bacteria oxidizing molecular hydrogen. Leeuwenhoek ned. Tijdschr 8:71
    [Google Scholar]
  5. Kluyver A. J., Verhoeven W. 1954; Studies on true dissimilatory nitrate reduction. 4. On adaptation in Micrococcus denitrificans. Leeuwenhoek ned. Tijdschr 20:337
    [Google Scholar]
  6. Packer L., Vishniac W. 1955; Chemosynthetic fixation of carbon dioxide and characteristics of hydrogenase in resting cell suspensions of Hydrogenomonas ruhlandii nov. spec. J. Bast 70:216
    [Google Scholar]
  7. Schatz A., Bovell C. 1952; Growth and hydrogenase activity of a new bacterium, Hydrogenomonas facilis. J. Bact 63:87
    [Google Scholar]
  8. Virtanen A. I., De Ley J. 1948; The enzyme activity and nitrogen content of bacterial cells. Arch. Biochem 16:169
    [Google Scholar]
  9. Wilson E., Stout H. A., Powelson D., Koffler H. 1953; Comparative biochemistry of the hydrogen bacteria. I. The simultaneous oxidation of hydrogen and lactate. J. Bact 65:283
    [Google Scholar]
  10. Wisseman C. L., Smadel J. E., Hahn F. E., Hopps H. E. 1954; Mode of action of chloramphenicol. I. Action of chloramphenicol on assimilation of ammonia and on synthesis of proteins and nucleic acids in Escherichia coli. J. Bact 67:662
    [Google Scholar]
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