1887

Abstract

SUMMARY: The amount, type and properties of nucleic acid have been estimated in the two end fractions, Ryan supernatant (Ryan Sup.) and Ryan final filaments (Ryan F.F.) of a purification procedure applied to allantoic fluid preparations of filamentary Ryan virus. Ryan Sup. consists of 80 % spherical particles and 20 % short filaments (length:diameter < 6). Analysis indicates an average nucleic acid content of 0·75 % (PR 8 = 0·91 %). The value of the ratio, adenine+uracil: guanine + cytosine for the nucleic acid of A strains of influenza virus varies between 1·22 and 1·28. The value for Ryan Sup. is 1·20; for a mutant strain of Ryan virus which exists almost exclusively as spheres, the value is also 1·20.

The ratio of filaments to spherical particles in Ryan F.F. is about 50:50. On a dry-weight basis, Ryan F.F. contains about 0·25 % RNA. There may be small amounts of DNA present. On a particle basis, the residual filamentary structures contain about eight times as much RNA as do PR 8 spheres. The value of the above ratio of bases however is about 0*9. Treatments of filaments with diethyl ether releases a soluble complement-fixing antigen (CFA) which on purification is found to have the same nucleic acid content per unit of CF activity as does soluble CFA isolated from either PR 8 or Ryan Sup. viruses. In each case the value of the above ratio of bases is about 1·25. On a particle basis filaments in Ryan F.F. contain 3 to 4 times as much soluble CFA as does PR 8 virus so that the residual RNA in Ryan F.F. must closely correspond in properties to RNA from the potential host cell which has a value for the above ratio of about 0·6. Exposure of Ryan F.F. to ribonuclease or to a procedure which degrades the filamentary form to smaller spherical units does not affect the amount or properties of the associated RNA.

A tentative scheme is proposed for the formation of virus particles of Ryan F. preparations. It is postulated that most of the spheres present in such preparations arise by fragmentation of the tip of forming filaments where there is a relative concentration of viral type RNA. The filaments which are found in the allantoic fluid thus represent only part of the original filamentary structures. This concept implies that filaments break more readily at those places where there is an enrichment of viral type nucleic acid.

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1958-08-01
2022-05-16
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References

  1. Ada G. L. 1957; Ribonucleic acid in influenza virus. The Nature of Viruses p. 104 Ciba Foundation Symposium London: Churchill;
    [Google Scholar]
  2. Ada G. L., Gottschalk A. 1956; The component sugars OF the influenza virus particle. Biochem. J 62:686
    [Google Scholar]
  3. Ada G. L., Perry B. T. 1954; The nucleic acid content of influenza virus. Aust. J. exp. Biol. med. Sci 32:453
    [Google Scholar]
  4. Ada G. L., Perry B. T. 1955; Specific differences in the nucleic acids from A and B strains of influenza virus. Nature; Lond: 175854
    [Google Scholar]
  5. Ada G. L., Perry B. T. 1956; Influenza virus nucleic acid: relationship between biological characteristics of the virus particles and properties of the nucleic acid. J. gen. Microbiol 14:623
    [Google Scholar]
  6. Ada G. L., Perry B. T., Abbot A. 1958; Biological and physical properties of the Ryan strain of filamentous influenza virus. J. gen. Microbiol 19:23
    [Google Scholar]
  7. Archetti I. 1954; Appearance associated with filamentous forms of influenza virus. Arch. Virusforsch 6:29
    [Google Scholar]
  8. Burnet F. M. 1956; Structure of influenza virus. Science 123:1101
    [Google Scholar]
  9. Burnet F. M., Lind P. E. 1957; Studies on filamentary forms of influenza virus with special reference to the use of dark-ground-microscopy. Arch. Virusforsch 7:413
    [Google Scholar]
  10. Chargaff E., Zamenhof S. 1948; The isolation of highly polymerised deoxy- pentosenucleic acid from yeast cells. J. biol. Chem 173:327
    [Google Scholar]
  11. Donald H. B., Isaacs A. 1954; Some properties of influenza virus filaments shown by electron microscopic particle counts. J. gen. Microbiol 11:325
    [Google Scholar]
  12. Donnelley M. 1951; Studies in experimental immunology of influenza. VII. An improved complement-fixation technique. Aust. J. exp. Biol. med. Sei 29:187
    [Google Scholar]
  13. Frisch-Niggemeyer W., Hoyle L. 1956; The nucleic acid and carbohydrate content of influenza virus A and virus fractions produced by ether disintegration. J. Hyg., Camb 54:201
    [Google Scholar]
  14. Frommhagen L. H., Knight C. A. 1956; The polysaccharide and ribonucleic acid content of purified influenza virus. Virology 2:430
    [Google Scholar]
  15. Keck K. 1956; An ultramicro technique for the determination of deoxypentose nucleic acid. Arch. Biochem. Biophys 63:446
    [Google Scholar]
  16. Knight C. A. 1947; Amino acid composition of highly purified viral particles of influenza A and B. J. exp. Med 86:125
    [Google Scholar]
  17. Martin E. M., Morton R. K. 1956; The chemical composition of microsomes and mitochondria from silver beet. Biochem. J 64:221
    [Google Scholar]
  18. Mayer M. M., Osler A. G., Bier O. G., Heidelberger M. 1946; The activating effect of magnesium and other cations on the haemolytic function of complement. J. exp. Med 84:535
    [Google Scholar]
  19. Morgan C., Rose H. M., Moore D. H. 1956; Structure and development of viruses observed in the electron microscope. III. Influenza virus. J. exp. Med 104:171
    [Google Scholar]
  20. Morzycki J., Taylor K., Kawecki Z., Wysoczynska H., Taylor A. 1956; Comparison of nucleic acid contents in complete and incompete forms of the influenza virus. Bull. Inst. mar. trop. Med. Gdansk 7:80
    [Google Scholar]
  21. Ogur M., Rosen G. 1950; The nucleic acids of plant tissues. 1. The extraction and estimation of deoxypentose nucleic acid and pentose nucleic acid. Arch. Biochem 25:262
    [Google Scholar]
  22. Schafer W. 1957; Units isolated after splitting fowl plague virus. The Nature of Viruses p. 91 Ciba Foundation Symposium London: Churchill;
    [Google Scholar]
  23. Schafer W., Zillig W. 1954; Über den Aufbau des Virus-Elementarteilchens der klassischen Geflügelpest. Z. Naturf 9b:779
    [Google Scholar]
  24. Valentine R. C., Isaacs A. 1957; The structure of influenza virus filaments and spheres. J.gen. Microbiol 16:195
    [Google Scholar]
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