1887

Abstract

SUMMARY: An influenza A strain, Ryan, isolated in Melbourne in 1954 contains on a particle basis about 20% filaments. By using the electron microscope for particle counting, the efficiency as haemagglutinin of Ryan ‘long’ filaments (particles in which length: width ⩾ 6) was estimated to be about 6 times greater than that of spheres of PR 8 virus. Allantoic fluid preparations of Ryan virus have high values for the ratio (log) EID50:AD and estimates indicate that a maximum of 6 ‘long’ filaments are equivalent to one EID50, compared with 18 particles in the case of PR 8 virus or 14 particles in the case of a mutant strain of Ryan virus which exists almost exclusively as spheres. Compared with virus PR 8 spheres (16 particles: AD), spherical particles present in filamentous Ryan preparations have a low efficiency as haemagglutinin (29 to 38 particles:AD). These particles are formed before the ‘long’ filaments, and in fluids harvested 21 hr. after inoculation when the proportion of ‘long’ filaments is very low, about 9 spherical particles constitute 1 EID50. For the purpose of subsequent chemical examinations filaments were isolated and concentrated by a process involving specific adsorption to and elution from red cells, followed by 3 cycles of differential centrifugation. The ratio of filaments: spheres in such preparations is about 50:50. ‘Purified’ filaments weigh about 30 times as much as the spherical particles present and account for over 95 % of the total weight present in purified preparations.

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/content/journal/micro/10.1099/00221287-19-1-23
1958-08-01
2022-01-27
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References

  1. Ada G. L., Perry B. T. 1954; The nucleic acid content of influenza virus. Aust. J. exp. Biol. med. Sci 32:453
    [Google Scholar]
  2. Ada G. L., Perry B. T. 1956; Influenza virus nucleic acid: relationship between biological characteristics of the virus particle and properties of the nucleic acid. J. gen. Microbiol 14:623
    [Google Scholar]
  3. Ada G. L., Perry B. T., Edney M. 1957; Infectivity of influenza virus filaments. Nature; Lond: 1801134
    [Google Scholar]
  4. Bang F. B., Isaacs A. 1957; Morphological aspects of virus cell relationship in influenza, mumps and Newcastle (myxovirus). The Nature of Viruses p. 249 Ciba Foundation Symposium London: Churchill;
    [Google Scholar]
  5. Burnet F. M., Lind P. E. 1957; Studies on filamentary forms of influenza virus with special reference to the use of dark-ground-microscopy. Arch. Virusforsch 7:413
    [Google Scholar]
  6. Burnet F. M., McCrea J. F., Stone J. D. 1946; Modification of human red cells by virus action. 1. The receptor gradient for virus action in human red cells. Brit. J. exp. Path 27:228
    [Google Scholar]
  7. Chu C. M., Dawson I. M., Elford W. J. 1949; Filamentous forms associated with newly isolated influenza virus. Lancet i:602
    [Google Scholar]
  8. Donald H. B., Isaacs A. 1954a; Counts of influenza virus particles. J. gen. Microbiol 10:457
    [Google Scholar]
  9. Donald H. B., Isaacs A. 1954b; Some properties of influenza virus filaments shown by electron microscopic particle counts. J. gen. Microbiol 11:325
    [Google Scholar]
  10. Fisher R. A. 1954 Statistical Methods for Research Workers, 12th ed. London: Oliver and Boyd;
    [Google Scholar]
  11. Francis T. 1934; Transmission of influenza by filterable virus. Science 80:457
    [Google Scholar]
  12. Francis T. 1940; New type of virus from epidemic influenza. Science 92:405
    [Google Scholar]
  13. Hirst G. K. 1952; Strain-specific elements in influenza antigens. J. exp. Med 96:589
    [Google Scholar]
  14. Horsfall F. L. 1954; On the reproduction of influenza virus. Quantitative studies with procedures which enumerate infective and haemagglutinating virus particles. J. exp. Med 100:135
    [Google Scholar]
  15. Isaacs A. 1957; Particle counts and infectivity titrations for animal viruses. Advanc. Virus Res 4:111
    [Google Scholar]
  16. Ledinko B., Perry B. 1955; Studies with influenza B of recent human origin. 1. Adaption to the mouse lung. J. Immunol 74:371
    [Google Scholar]
  17. Morgan C., Rose H. M., Moore D. H. 1956; Structure and development of viruses observed in the electron microscope. III. Influenza virus. J. exp. Med 104:171
    [Google Scholar]
  18. Oster G. 1946; Molecular weights and other properties of viruses as determined by light absorption. Science 103:306
    [Google Scholar]
  19. Tyrrell D. A. J., Valentine R. C. 1957; The assay of influenza virus particles by haemagglutination and electron microscopy. J. gen. Microbiol 16:668
    [Google Scholar]
  20. Valentine R. C., Isaacs A. 1957; The structure of influenza virus filaments and spheres. J. gen. Microbiol 16:195
    [Google Scholar]
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